FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_HTO3_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_HTO3_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44072100
Sequences flagged as poor quality0
Sequence length116
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1368990.3106250893422369No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAC8952000.0109.499331
CATGTTA8072750.0109.395882
ACATGTT8097450.0109.249151
AGGACCA7796600.0109.2327651
TTACTAT8970750.0109.228944
TATCCCC8872150.0109.1897058
TCGATAA4263300.0109.187521
TACTATC8938700.0109.153735
TGTTACC8056750.0109.110094
CTTACTA8984500.0109.108983
GCTTACT8974900.0109.1002962
CAAGAAC14069950.0109.097847
ATCCCCC8822100.0109.087159
CTATCCC8886150.0109.07467
ATGTTAC8084450.0109.055043
CCGTCCC7968650.0109.039989
CCATCCA7796900.0109.016095
CATCCAA7782000.0109.012476
GGACCAT7802900.0108.971852
ACGGAAC14132300.0108.970811
Produced by FastQC (version 0.11.5)