FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_GDO2_0824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_GDO2_0824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16525
Sequences flagged as poor quality0
Sequence length116
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12097.316187594553707No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2381.4402420574886534No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1050.6354009077155824No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.38729198184568836No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.3388804841149773No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.29046898638426627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG380.22995461422087746No Hit
AAGAAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT240.14523449319213314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1700.094.385221
AGCAGTG1800.089.14162
GCAGTGG2450.065.094873
GTATCAA2550.064.698759
AGTGGTA2500.063.792975
CAGTGGT2500.063.792974
GGTATCA2600.063.4545448
TGGTATC2600.061.339397
GTGGTAT2650.060.182056
GGGAATG150.00573663854.9939462-63
TACTTTT2300.037.06113422-23
ATCAACG2450.035.9144110-11
CAACGCA2400.035.5169212-13
ACGCAGA2350.035.10251214-15
AGAGTAC2350.035.10251218-19
GTACTTT2250.034.21845222-23
AGTACTT2350.033.9324320-21
CTTTTTT2600.033.84242224-25
GCAGAGT2450.032.54743616-17
TCAACGC2450.032.54743612-13
Produced by FastQC (version 0.11.5)