FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_ADT1_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_ADT1_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49622451
Sequences flagged as poor quality0
Sequence length116
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1558970.3141662631698704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTC8531100.0109.775921
CCCGTCC8544050.0109.630742
CCGTCCC8530000.0109.577263
CGTCCCT8536050.0109.522824
CGTAGTC6127850.0109.43472
TTAGAGT12170450.0109.4176943
TAGAGTC12202300.0109.2818454
TTCCTCA3491300.0109.2299042
CCCTAAG8582450.0109.21877
CCTCATT3494750.0109.205344
TCCCTAA8590600.0109.189256
GTCCCTA8598500.0109.108655
TCTGCCA12113200.0109.016239
AGAGTCT12227150.0108.973265
AGTCTGC12175350.0108.93277
CATATCC4958750.0108.918796
GAGTCTG12217600.0108.9179466
TGCCATA4981600.0108.9031753
GTCTGCC12145550.0108.8830648
TGGCCGA4194400.0108.755466
Produced by FastQC (version 0.11.5)