FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n02_ADT1_0824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n02_ADT1_0824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162
Sequences flagged as poor quality0
Sequence length116
%GC20

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7345.06172839506173No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116.790123456790123No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53.0864197530864197No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42.4691358024691357No Hit
AAGCAGTGGTATCAACGCAAAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
GCATAGCTCTTAAACAGCGACACGGTGGGGTTTCCCCCCATATAAGAAAT10.6172839506172839No Hit
AAGCAGTGCTAACAACGCAGAGTAATATTTTTATTTTTTTTTTTTTTTTT10.6172839506172839No Hit
CTATAGACTCACTGACCCCGTTAGGACAGCGGCGAACCAATTCCGGTAAC10.6172839506172839No Hit
GCATAGCTCTTAAACGACGGTGTGCTCGCTTTCCACCCCATATAAGAAAG10.6172839506172839No Hit
CAATAGATTCTGCAACCAATATGACCAAACGTTGCCCACCTAAAAGACCA10.6172839506172839No Hit
ACGTAGTCACCTAGTCCCATATAAGAAACGATTATTCTAGCAGATCTTTG10.6172839506172839No Hit
ACAAAGACCAAAAAACCCATAAAACAAATATAATATTATCAAACACTATA10.6172839506172839No Hit
GCAGTGGTATCAACCCAGAGTACATTTTTTTTTTTTTTTTTTTTTTTATT10.6172839506172839No Hit
AAGCAGTGGTATCAACGCAGAGTACGTTTTTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
GCATAGCTCTTAAACCGTTCGGACGAGAAGCCAGACCCCATATAAGAAAG10.6172839506172839No Hit
GCATAGCTCTTAAACACGCACGTGTCTGTGTAGGGCCCCATATAAGAAAT10.6172839506172839No Hit
GCATTGATTTTTGGCGCCCTAAAGGAAAGTTAGGCCCACAGATTAGCACG10.6172839506172839No Hit
ACATAGAAAAAAAAAACCAAGAAGCAAATCTTCTCACCACAAAAAATAAA10.6172839506172839No Hit
CCGCACAAAATCCGACCCATATAAGAAAGCGGAAGCGGAGTCCGAGAGGT10.6172839506172839No Hit
ACGTAGTCACCTAGTCCCATATAAGAAATCGAACTATGCCTTACTCTTAG10.6172839506172839No Hit
GCATAGCACTTAAAAGCAAGTTCGTTATGTCACCCCCCCAAATAAAAAAT10.6172839506172839No Hit
GCAAATCACTAAAAACCTATAAATCCAACTAGACAACAAAATAACAAAGA10.6172839506172839No Hit
CTGCTTTCCTCAACACCACATCAGCATATCTTCGGCCCACACCCTTAATG10.6172839506172839No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
AGGGGGGGGGGTGAAAGCAGACGAAGGGTTTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
GCATAGCTCTTAAACTAACTGATACTGAAGCTGACCCCCATATAAGAAAT10.6172839506172839No Hit
GTATTGGTGACTAAATATGTTGTCTAGAGTTCATGGGAGATTGCGTCATA10.6172839506172839No Hit
AAGCAGTGGTATCAACGCAGAGTAGTTTTTTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.6172839506172839No Hit
CAACGGCAGATAAACCTGAATAATAAACACTCATGAATGACCTGACTACA10.6172839506172839No Hit
GAAAAGCTCTTAAACCCGATGCAACAAGGACGTAACCACATATAAGAAAT10.6172839506172839No Hit
AAGCAGTGGTATGAACGCAGAGTAGTTTTTGTTTTTTTTTTTTTGTTTTT10.6172839506172839No Hit
CTGGATCTTGGCCTTTACATTTTCTATCGTATCCGAGGGTTCAACCTCTA10.6172839506172839No Hit
CACAGCAACCAAGAATCGATAGACTCAATGCAAACTGCCCTCATTTAAAA10.6172839506172839No Hit
CTGCGCCCGCTCATAAGGGGATGGCCATGGCTAGCTTTATAGATAGTTGG10.6172839506172839No Hit
AAAAAGTCAAATAAACCCATATAACAAAATTTATTTAATAATTTAAAATA10.6172839506172839No Hit
AAAAATCACTAAAACTAGAATAGAGAAAAGAGATGCCACAAATAACAAAT10.6172839506172839No Hit
ATCACCGTTAATAAACACAACTTCATTCATCATCAATGTGCTACTACAGC10.6172839506172839No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTATTTTTTTTTT10.6172839506172839No Hit
GATCTCGGCTCACTTCCGGCTCCACGTAATGAGGAATGAGAATTTGCTTG10.6172839506172839No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTGTT10.6172839506172839No Hit
ACGCAGTATTTCCGACCCATATAAGAAAGCGCCTAAAATAGACAGCTAGT10.6172839506172839No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTGTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
ATTTGTCTACTTGGCTACACCACTGGCTTAGCTGCCAAGAGCTCAATGTC10.6172839506172839No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTA10.6172839506172839No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.6172839506172839No Hit
ATAACTATTAGTGGCAGGTTAGTTGTTTGTAGGGCTCATGGTAGGGGTAA10.6172839506172839No Hit
ACGTAGTTACCTAGTCCCATATAAGAAACGTGCTGTTTTTACCTTGAACC10.6172839506172839No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTATTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
AAGAAGATGGCCACCCCTCAAAAACAAATCAATATCATGTCTCCTTAAAT10.6172839506172839No Hit
ACACGTCAAAAAGAACCCATATAACAAAAACACTTAAAAACATCACTACA10.6172839506172839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTATTTTTTT10.6172839506172839No Hit
ACGCAGTATTTCCGACCCATATAAGAAATCTCACTTTACGGTACTCTGCG10.6172839506172839No Hit
CACCCTGGGTTGTCGGAATCGCTGACTACACGGGAATTCACTGTGCGACC10.6172839506172839No Hit
GTTCCGCGAGCTCAAATCGGATAACAACCACTGACACACTCATAAGGCAG10.6172839506172839No Hit
ACATAGTCACCTAGTCCCATATAAGAAAATAATTAACCATAGTTATGTGA10.6172839506172839No Hit
GCATAGCTCTTAAACCAACTGATCACATCACGATCCCCCATATAAGAAAG10.6172839506172839No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.6172839506172839No Hit
CTCCATGACGGGGATATCCACGCCCTAACCCACCACACACTCCAGCTGGA10.6172839506172839No Hit
CATTAGAGTCTGCCACCCATATAAGAAAAACCAAACTTGGTCGCTGACCA10.6172839506172839No Hit
CAACTTGGCAGAATCCCCATATAAGAAAAGTGCTACTGAGAAAAGCGAAG10.6172839506172839No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGTGGGTGGGGG10.6172839506172839No Hit
GCATAGCTCTTAAAACCCTCACATCCTGAACAATACCCCCATATAAGAAA10.6172839506172839No Hit
ATACACAGCAAACATGCGGCACAGCTGGAGAGGCATGGAACCCAGCTGGC10.6172839506172839No Hit
GCATAGCTCTTAAACCAATTGAATGCTACATCTAACCCCATATAAGAAAA10.6172839506172839No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTATTTTTTTTTTTTTTT10.6172839506172839No Hit
ACCCGTCCCAAAGAACCCATATAAGAAAATCCCTCGCTAACGCTCCTTAA10.6172839506172839No Hit
AAAAAGATCTATAAAATAAGAGTACATTTAATATAAAAATTATAATAATA10.6172839506172839No Hit
AAGCAGTGGTATCAACGCAGAGTACTGTTTTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6172839506172839No Hit
ACTGCCATATCCCTACCCATATAAGAAAACAGATCCAACGCGACAACACT10.6172839506172839No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC100.0036472713110.07
AGTGGTA100.0036472713110.05
GTATCAA100.0036472713110.09
AGCAGTG100.0036472713110.02
GCAGTGG100.0036472713110.03
GTGGTAT100.0036472713110.06
CAGTGGT100.0036472713110.04
GGTATCA100.0036472713110.08
AAGCAGT100.0036472713110.01
Produced by FastQC (version 0.11.5)