FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6384092
Sequences flagged as poor quality0
Sequence length26
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG92390.1447190923940319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT10600.017.1760771
GGGACCT8150.017.0614381
CTGACAC10150.017.0449434
ATGGGAG9400.017.0274871
GACCTGG7350.016.8769041
GGCCCTA9950.016.87576510
GACTGCG12350.016.767251
AGCTGAA7400.016.7481678
CGGAGCT12000.016.6727491
CATGGAG3500.016.572023
TCCCGAT9250.016.5465761
CACTGAG5400.016.4825862
CCTCTGA16650.016.2221341
TCTGACA11400.016.1409243
TGCGGGT7600.016.0584891
TCGGTAA13350.016.035811
TGAAGCT6800.016.0299845
AAGGGAA6300.016.0235310
CCGGGAT6750.016.0058381
TCGGGCA7150.015.9446243
Produced by FastQC (version 0.11.5)