FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_cDNA4_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_cDNA4_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466408
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTGG8850.017.5188751
TGACGGC9150.017.4910811
GGGACCT8650.017.4613841
GAATGAA9700.017.4274061
TGCGGGT8850.017.405851
GGGAATG6900.017.396021
TACTTGT9250.017.3019891
ATGCGAT8650.017.114471
CAATGGA14700.017.0740438
AGCTACC8250.016.9689148
ACTGAAC9100.016.9276681
GGAAAGC8400.016.9093481
GACTGCG10000.016.9045851
TGCCAAA10200.016.867321
AGGACGA4750.016.8413288
GATGCTA8650.016.7675531
CGCTGGA7100.016.765111
CGCTATC11800.016.6994441
GGATTAC9000.016.6711881
ACTGATG8300.016.6310181
Produced by FastQC (version 0.11.5)