FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_cDNA3_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_cDNA3_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5731257
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA22100.017.5107388
CTGCGGA11600.017.5072151
AGCTTGA21250.017.4660221
GGGACCT14550.017.3954871
ATGCGAT14350.017.3590721
GCGCGAT10650.017.1901861
GAATGAA15500.017.1683671
GGGAATG10500.017.1499251
ATGGGAG17950.017.1101171
TGCGGGT14800.017.1016451
GGCGACT15650.017.0038151
GAAATGA16000.016.9444871
AGGATGG8450.016.9225238
GGAAAGC13500.016.8958551
GACCTGG15050.016.8840351
AGGACGA6300.016.8248488
GGGAGAT12850.016.816271
GGACGAA6300.016.6661229
ACGCCGA19950.016.598321
GATGCTA15850.016.5367851
Produced by FastQC (version 0.11.5)