FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_cDNA2_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_cDNA2_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5584290
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA20400.017.7443188
ATGCGAT15250.017.7112771
GGGACCT13800.017.397551
TCCCGAT17900.017.1571
ATCGAGT17400.017.132591
ACTGAAC14750.017.0908831
TGCGGGT13300.017.0738011
CCGGGAT10350.017.0109391
CTGAAGT15900.016.987231
GGATTAC16900.016.9291571
GGGAGAT13550.016.9064391
GATGCTA15000.016.8060341
GATGAAA11700.016.7581541
GAATGAA15350.016.7486861
GGGAATG9050.016.6910761
GACCTGG15350.016.6835161
GAAATGA14950.016.66151
AGCTTGA19550.016.5276741
ACGCCGA17650.016.4931741
ACTGATG12100.016.4521881
Produced by FastQC (version 0.11.5)