FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_cDNA1_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_cDNA1_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052871
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC6950.018.135171
GAATGAA8200.017.8104481
TGGACGC9000.017.783341
GACCTGG7650.017.783341
TGCGGGT7100.017.7520331
CCGGGAT6500.017.6978451
CGGACTG6500.017.5439511
ATGAATG5500.017.45371610
GGGAGAT7600.017.2422371
CTGCGGA6500.017.2361621
CAATGGA10100.017.12798
GGAAAGC6950.017.127661
TGCGTGG6900.017.10681
TGCCAAA9600.016.984481
GGGACCT7600.016.9789961
GATGAAA5600.016.9695951
AGGACGA3450.016.8107978
ACTGAAC8100.016.7953761
ATCGAGT9000.016.7830281
ATTGGTG9500.016.742081
Produced by FastQC (version 0.11.5)