FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_cDNA1_0824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_cDNA1_0824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATTGAGCCGTCGTTTTTGTCACG12.857142857142857No Hit
GGAACTTAGCAAGGATAGCTTAATAC12.857142857142857No Hit
GCATACAGTTGCTCCTCGAGGTTTCG12.857142857142857No Hit
TTCCCAGAGGTGATATTGCTTGCACT12.857142857142857No Hit
ACATACGTCGCTTAGACTAACCTTAA12.857142857142857No Hit
ACATACGTCGCTTAGATGGGGCGCAT12.857142857142857No Hit
ACGCCGAGTACCGGCTGTCATCCGGA12.857142857142857No Hit
CGTTCTGGTCCATGATGTGACACTGA12.857142857142857No Hit
AGGGATGTCGTGGACCTCAATGTTCT12.857142857142857No Hit
GAAACTCTCCGCGTTTAAACATCCCG12.857142857142857No Hit
AGCAGCCCAGTGGAGTCCATTTCCTG12.857142857142857No Hit
CAACCAACATGGATGGGTATCGTGTT12.857142857142857No Hit
ACGGCCACAATGAATGCCCCAATTCG12.857142857142857No Hit
CTAGTGATCGCCGTGACAACTTGCAT12.857142857142857No Hit
CTGATCCGTGACAAATCTCGTATGCC12.857142857142857No Hit
CCCAATCGTTGCTCCTGGAAACCCGG12.857142857142857No Hit
CATCCACTCCCGACTTTAAACCACGG12.857142857142857No Hit
TCAGGATAGGTGCTTTGCATTTTTCA12.857142857142857No Hit
TTCCCAGGTTAAGATGCTCCTACGTT12.857142857142857No Hit
TAGGCATAGCACACAGGAGTATTTTC12.857142857142857No Hit
ATTTCTCAGACAGGCATCACTCGCAA12.857142857142857No Hit
ACCCACTAGAGCTGGTGCTAACAACT12.857142857142857No Hit
GTCAAGTCACTAGTACACATACGCTT12.857142857142857No Hit
CATGGCGAGCTAGTCTTTCAATCAAC12.857142857142857No Hit
CGTTGGGTCAGTGCATCCGATGCTCG12.857142857142857No Hit
AGCGTATTCCAATGGTTGTGTTCTCT12.857142857142857No Hit
GGTATTGAGTACGTAATCAGCTCCGC12.857142857142857No Hit
GCGGGTTTCCCTTGTGCGGGTGTCTT12.857142857142857No Hit
GGTGCGTCAATGAATGTTTCATAACC12.857142857142857No Hit
GAACCTATATTGGCTACACTGACACT12.857142857142857No Hit
GCCAAATGTACCGGCTTCCTAATTGT12.857142857142857No Hit
GCATGATTCCGCGTTTATTTAAGACT12.857142857142857No Hit
GCGGAGAGTACCGTATCCGCAGACGT12.857142857142857No Hit
GCACATAAGTGGGCTATGGGCACTGA12.857142857142857No Hit
GAATAAGCATGGAATACTGCGGTCAC12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)