FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_HTO3_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_HTO3_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44072100
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA214500.018.8166075
TCCCGTA219950.018.7641094
CTCCCGT239350.018.3671463
AACTCCC292850.018.3088131
AAATGTG245400.018.29169810
ACTCCCG241850.018.1859682
GTAAATG272400.017.9837348
GGCCCTA114300.017.9431410
TAAATGT251550.017.824649
CGTAAAT250800.017.4114977
CAATGGA124950.017.3981388
TTGACGA144950.017.34997210
CATGGCG170450.017.2951281
ATAAGAG284550.017.1941091
TTGAACG118100.017.160511
GGGACCT88150.017.1581361
GCTGCAG150950.017.1039141
GAATGAA107000.017.0896451
CTGAAAC107000.017.0709481
ACTGAAC115300.017.0393431
Produced by FastQC (version 0.11.5)