FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_HTO3_0824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_HTO3_0824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length26
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGGTGCAGACAAAAAGTCTGG11.0869565217391304No Hit
GGAAAGCAGAGAACAGCGTTGTACAA11.0869565217391304No Hit
GGGCACTCAAGGTGTGACTTAGTGGC11.0869565217391304No Hit
TCGTACCTCGCGCCAAAAGTTTTTCC11.0869565217391304No Hit
AGGGTGAGTCTGCCAGAACAATTTTG11.0869565217391304No Hit
CTGTTTAGTCACCTAATTTTTATAGG11.0869565217391304No Hit
GCTGCAGCAAGTCATCCCTTATTATC11.0869565217391304No Hit
CGTGAGCTCCAGAAGGCCCTGTAACG11.0869565217391304No Hit
CGGCTAGCATCACAACATGGAGCACT11.0869565217391304No Hit
GCAATCAAGCCACGCTTACTTAGGAG11.0869565217391304No Hit
AGCACCAACAGAAACACCCTCATTAG11.0869565217391304No Hit
AGCGTATAGGCAGTCAAGGCATATCT11.0869565217391304No Hit
GCGCAACGTGCAGGTATACGGATCTA11.0869565217391304No Hit
ACATGGTAGTTAAGTGCTCCTTTCGA11.0869565217391304No Hit
GATCAGTCAGGAACGTCCATTCTAAT11.0869565217391304No Hit
GCAGTTGGTTGGGGTGTCCTGTCGCT11.0869565217391304No Hit
ATTCTACCACCCAGTGCCCACCTGTC11.0869565217391304No Hit
CGTCAGGCATACTACGCAGAGGCCCA11.0869565217391304No Hit
ACGGAGAGTTGGGACACGATGAGCGG11.0869565217391304No Hit
TCACAAGCACAGCCCACCCGGGTTGC11.0869565217391304No Hit
GCGGGACTCGGCGCTATGGGTACGGG11.0869565217391304No Hit
GGCAATTTCCAGAGGAAATACTCTTA11.0869565217391304No Hit
CACCTTGCAAACCCATCAGGCCTACA11.0869565217391304No Hit
AACTGGTAGCTGATAAGCCTTAGGGC11.0869565217391304No Hit
CGGACGTAGACAGAGACCACGGTAAA11.0869565217391304No Hit
TCACAAGTCTGGTTCCGGGTATCCAT11.0869565217391304No Hit
GCTGCTCAGCACCGCTGCAAAACGTT11.0869565217391304No Hit
GTCTCGTACTGAGCAGGATTACCATG11.0869565217391304No Hit
GAAATGAGTACCGAGACCGTGGACTG11.0869565217391304No Hit
TGTATTCAGCAGCCTCACACTGCCGA11.0869565217391304No Hit
TAAGTGCCATGTCTCCCGCCCTTTCG11.0869565217391304No Hit
GTTTCTAGTATAGGTACGCGTACGAC11.0869565217391304No Hit
TGTCCCAAGCTGAAATACCAACCGTC11.0869565217391304No Hit
CGAACATGTCCGAAGAATTCAAGCCT11.0869565217391304No Hit
CAGCTAACAGTAGAGCGTGTAAACTT11.0869565217391304No Hit
AAATGCCAGTACGATATTACAGCTGC11.0869565217391304No Hit
CCGTACTCCGATCTCCGTACTCATAT11.0869565217391304No Hit
TAAGAGACATAGAAACACAGGGCATC11.0869565217391304No Hit
GGGGTTGCAAATCCGTGAAGTCTGAG11.0869565217391304No Hit
ACTATCTCATTTGCTTAGCGGTCCGC11.0869565217391304No Hit
GAAGTGCTCATGAAGTCGGGCCGCCC11.0869565217391304No Hit
CGTGTCTTCATACGGTGCTGGTACTT11.0869565217391304No Hit
GGGAATGCACGGTAAGTTGCTACTCA11.0869565217391304No Hit
GTTTCTAGTCTTGCGGTCACGGTTCA11.0869565217391304No Hit
CGATCGGTCATGTAGCAAACGTACCT11.0869565217391304No Hit
GTATCTTGTAAGGGCTATCATGTGGA11.0869565217391304No Hit
GAAATGATCCATGAACACACCTAACT11.0869565217391304No Hit
AGCTTGAGTTGGAGGTAAGAAGGGTC11.0869565217391304No Hit
GGCTTGCCACGTGGCGCGAGGCTGAC11.0869565217391304No Hit
GTTGGTGGTGAACCTTTGGCGGGTAT11.0869565217391304No Hit
AGTGGGAAGAAGCCCACAACGCATTA11.0869565217391304No Hit
TGACTTTCAGGCGATAGGAAGGGGCG11.0869565217391304No Hit
CTAAGACAGCTGCCCACGCGACGCCG11.0869565217391304No Hit
TATCAGGAGAATTCCCGCTCCACAGC11.0869565217391304No Hit
TTCGGTCGTGCGGTAAGTCCAAGGGA11.0869565217391304No Hit
GAGCAGAAGGCTAGACCATATCCTGT11.0869565217391304No Hit
CAGCTGGAGAATTCCCGATATTCGTA11.0869565217391304No Hit
TCGTAGATCCAGTATGTTGCCCCCCT11.0869565217391304No Hit
GTGGGTCGTTATGTGCTACTGTCTGT11.0869565217391304No Hit
AACTCTTGTACCGAGACCCGGGAGTC11.0869565217391304No Hit
TGCCAAAGTGGTAACGAAATCATTTT11.0869565217391304No Hit
CGTAGCGAGTGGGTTGAACCGTCGAG11.0869565217391304No Hit
CTAGAGTCAGTACACTACATACTGAA11.0869565217391304No Hit
ACTGAGTTCCTTTCGGAATACATCTA11.0869565217391304No Hit
GCATGCGGTGTTTGTGTCCAGACGGT11.0869565217391304No Hit
TCAACGATCAAACCGTGTCTTTCTTC11.0869565217391304No Hit
TGTTCCGTCAATCTCTTTAACTTGAC11.0869565217391304No Hit
ACACCAAAGGTGTTAAACTTCTACAC11.0869565217391304No Hit
TCCCGATAGCGTGTCCTGCACAATGT11.0869565217391304No Hit
GGATGTTGTCTAAAGACCCGGTTGGA11.0869565217391304No Hit
AGCTCCTTCCCGGATCAGCTGTGCGG11.0869565217391304No Hit
CACTCCAGTCCAACTACGTGTGACGT11.0869565217391304No Hit
GATCAGTGTTATGTGCCATCAGTAGA11.0869565217391304No Hit
GAACGGATCGCTGATAGATACTATTC11.0869565217391304No Hit
GTGAAGGCATGTCTCCCCAATGCACC11.0869565217391304No Hit
TGACTTTTACTTCCTCTAGATAGTCA11.0869565217391304No Hit
CACATTTTCAGAGGTTGCAGTGAGCT11.0869565217391304No Hit
GTGAAGGCAGTAAGCGCGCTTAACAA11.0869565217391304No Hit
CTCGAAAGTAAGAGGAGCTGGTGTCG11.0869565217391304No Hit
GATGCTAGTCAGAGGTGTGTGGGGTT11.0869565217391304No Hit
GTAGGCCGTAGTACCTCGGACTCGCG11.0869565217391304No Hit
TCACGAAGTGGTGTAGCATGTTGGGA11.0869565217391304No Hit
GATGAAAAGGGATCTGCACCATACGC11.0869565217391304No Hit
CACAAACTCCTGTAGATCGTACTCCT11.0869565217391304No Hit
GATCGTAAGTGGTAGCAGTGCTCCGC11.0869565217391304No Hit
CTCTGGTGCACCCACACTGCCCTCCC11.0869565217391304No Hit
CTGCTGGCAATCCCCTTTGTTGGCCC11.0869565217391304No Hit
CCACTACAGCTAGGCATCGCGATACA11.0869565217391304No Hit
ACCGTAAGTATGAATGGAGGCTAATT11.0869565217391304No Hit
AAACCTGGTTTGACACTTTAGACTCT11.0869565217391304No Hit
GCTGCAGTCAATCACGACCCTCACTG11.0869565217391304No Hit
GTGTGCGGTACGACCCAGGCTGTCCG11.0869565217391304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCT50.020.08
AATTTTT50.020.015
CCTAATT50.020.012
TTTTTAT50.020.017
TGTTTAG50.020.02
TTTTATA50.020.018
TTATAGG50.020.020
TAGTCAC50.020.06
GTTTAGT50.020.03
CACCTAA50.020.010
CTAATTT50.020.013
TAATTTT50.020.014
TTTAGTC50.020.04
TCACCTA50.020.09
CTGTTTA50.020.01
ACCTAAT50.020.011
TTTATAG50.020.019
ATTTTTA50.020.016
TTAGTCA50.020.05
AGTCACC50.020.07
Produced by FastQC (version 0.11.5)