FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_GDO2_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_GDO2_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58060072
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGA238550.017.7549671
ACTGAAC182450.017.6437381
CTAATGG259050.017.6396881
CTGACAC255900.017.6130434
TCTGACA270250.017.5992133
CGGACTG179650.017.4844061
TTGTAGG228750.017.4354671
TCTGGAA211800.017.4091591
CAATGGA170950.017.4078188
ATGGGAG195600.017.3934691
GGATTAC202350.017.3125631
GGCCCTA206100.017.30626510
TGACGGC175550.017.3058281
CCTCTGA374450.017.228481
AGCTTGA158450.017.2226731
CTCTGAC276900.017.2021312
GTTGATT238100.017.088698
TGCGGGT189100.017.0602991
ACTGATG141650.017.0548931
GATTCAG295350.017.0432361
Produced by FastQC (version 0.11.5)