FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_ADT1_0904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_ADT1_0904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49622451
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT216550.018.0664481
TCTGGAA216600.017.8775441
CACTGAG176250.017.7713072
CATGGCG242800.017.7570881
ATGGCGC172550.017.7408772
CAATGGA148500.017.6759688
TTGTAGG250250.017.6321851
TCGTCGC284900.017.5297556
ATGAATG153050.017.52280410
ACTGAAC170050.017.4359131
TGGACCC148400.017.43173810
GGCCCTA104700.017.37257610
CCTCTGA255400.017.3079761
ACACTGA299100.017.2775121
GTTGGAC150350.017.2256938
CCCAGAG155950.017.14733
CAAGGTC237350.017.144244
GGGAGAT135250.017.1146981
TCTGACA190800.017.086733
GCTGCAG165200.017.0819031
Produced by FastQC (version 0.11.5)