FastQCFastQC Report
Fri 4 Sep 2020
HGLKFAFX2_n01_ADT1_0824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLKFAFX2_n01_ADT1_0824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162
Sequences flagged as poor quality0
Sequence length26
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAATAAAAAAAAAAAAAAAAAAA21.2345679012345678No Hit
CATTATCAGGTTCCTATGGACTCCTA10.6172839506172839No Hit
GACTCTAAGAGTAAGGCATACTTCGA10.6172839506172839No Hit
CAAGTTGTCCGTCAAATAAGCCGACC10.6172839506172839No Hit
CTCTGGTTCAAGGTAAAAACAGCACG10.6172839506172839No Hit
ACAAATAAAAAAAAAAAAAAAAAAAA10.6172839506172839No Hit
TCGGTAACACCACCAGGTCTGTGTTA10.6172839506172839No Hit
CAAATCAACATCTATATAACAATATA10.6172839506172839No Hit
GTCTCGTGTTTAGCTGGAAGACGATA10.6172839506172839No Hit
AGAATAGGTGCTAGCCATTCTACAGG10.6172839506172839No Hit
AAAAAAGATTCAAGAAGTAAAAAAAG10.6172839506172839No Hit
TTGACTTAGAGCTATAGGCGGGCTGG10.6172839506172839No Hit
CTAATGGAGGGAAAAAAAAAAAAAAA10.6172839506172839No Hit
GCTGCGAAGATAGCATAAACAATTTA10.6172839506172839No Hit
ATCATGGCACATGGGATTCGGGAGTT10.6172839506172839No Hit
CGAGCCATCTACCTGCCTATCTCACT10.6172839506172839No Hit
TAGTGGTAGGACGAAATGTCTGTGTC10.6172839506172839No Hit
CACAGTAAGGCATGGTGGGGTCTACC10.6172839506172839No Hit
GTTCGGGGTGTTCGATACACACGCGC10.6172839506172839No Hit
GGGAGATAGCGCTTATGTCATTTTTC10.6172839506172839No Hit
CACATAGCAGAGTGTGGAGTGTGGAT10.6172839506172839No Hit
TCCCGATCATTTCACTAAACTTGGAA10.6172839506172839No Hit
CTACCCAAGGAATCGCTATGATCATA10.6172839506172839No Hit
ACATCAGTCGTTACAGATTGTGTAAT10.6172839506172839No Hit
GTGCAGCCATACTCTTTTACTTACGC10.6172839506172839No Hit
CAACCTATATGGAGACTCCTATTTAA10.6172839506172839No Hit
CAGCTAATCCCTGACTAGGACGACAT10.6172839506172839No Hit
GATCGTACATGCTAGTGGCAGTAATT10.6172839506172839No Hit
TGCACATTCGAGTTTCTCAGGAGCTC10.6172839506172839No Hit
GTGTGCGCAGTGGGATAGTTTTAATT10.6172839506172839No Hit
CCGACCACAAACGCGATCCTAAAGTC10.6172839506172839No Hit
AAGTCTGAGTGCTGCCGAGTATTTTA10.6172839506172839No Hit
TGAGGGACAAGGACACGTCCACTCTG10.6172839506172839No Hit
ACACCCTCAAAAAAAAAAAAAAAAAA10.6172839506172839No Hit
GTCACGGAGCCCGAAAAAAAAAAAAA10.6172839506172839No Hit
ACTGCTCCATCACGATTTAATCTTCA10.6172839506172839No Hit
TGACAACGTACGACCCTCGTTTTATA10.6172839506172839No Hit
CTTGGCTGTGCTTCTCCGCGCAATCA10.6172839506172839No Hit
ACACCAAACCTTAATCGCAAGTCAAC10.6172839506172839No Hit
GTTCAGCACGGTGTCAAAGTCGGCCT10.6172839506172839No Hit
ACTATAACATAACTATCAATAATTAT10.6172839506172839No Hit
AGCGTATGTATGCTTGGTAAAAGATA10.6172839506172839No Hit
TTCTACAAGCGAAATACATAGACTAT10.6172839506172839No Hit
GAACGGACACGAGAGTATATGAGGGT10.6172839506172839No Hit
CATCGAAGTCCAGTTACCAGTGCGTC10.6172839506172839No Hit
CTGAAGTCAACAACCTGACACGGACA10.6172839506172839No Hit
CTGGTCTGTAGATTAGTCATACTTTT10.6172839506172839No Hit
AGCCTAACATGCAACTTGCATCGTAA10.6172839506172839No Hit
CTACACCGTACGACCCTCTCGGCGTT10.6172839506172839No Hit
GCACTCTGTAAGTGTACAGCATTAAT10.6172839506172839No Hit
TTGGCAAAGATCTGCTAGAATAATCG10.6172839506172839No Hit
GGTATTGAGTACGTAATGTAATATTC10.6172839506172839No Hit
AAGGTTCTCAAACAAGGAAAAAAAAA10.6172839506172839No Hit
AAATGCCTCACAGTAAATCAAAACTC10.6172839506172839No Hit
CGAGCCAGTTGGTGGAATCATTCTTC10.6172839506172839No Hit
TTCTACATCAACACGTTTCTCTACTG10.6172839506172839No Hit
CACTTCTAAGGATTAGATTAAATTAT10.6172839506172839No Hit
CTACACCAGAACAACTAGTTATATAA10.6172839506172839No Hit
AAGGAGCCACAAGACGGATATGCGAA10.6172839506172839No Hit
CTCACACCAAAAAAAAAAAAAAAAAA10.6172839506172839No Hit
ACTTTCACACACATGTTCTTATCGCC10.6172839506172839No Hit
TCAGCAATCCTCGCATAATCGCCTTG10.6172839506172839No Hit
AACCAACATGGATGGTCATATGCCAT10.6172839506172839No Hit
ATTCTACCAAACTGTCGGTAACTCCT10.6172839506172839No Hit
ATCCACCTCTTCATGTCTCACGATTT10.6172839506172839No Hit
CACCTTGAGATAGTCATGGTCTCCGT10.6172839506172839No Hit
CACACTCTCTGATTTAAATAAACCTT10.6172839506172839No Hit
CTCGAGGCACACAGAGTCTTCAACGA10.6172839506172839No Hit
GCGACCAAGATGTTAGCGTCGCGCAA10.6172839506172839No Hit
CTGCGGACACAAAAAAAAAAAAAAAA10.6172839506172839No Hit
TGCGTGGTCAGCGACCAAGTTTGGTG10.6172839506172839No Hit
GGTGAAGGTGCAGTAGCTATGATATT10.6172839506172839No Hit
CATCGGGTCGAGAGCAAAAAGCCACC10.6172839506172839No Hit
TTAGGCAGTTGGACCCGTCCTGTTAT10.6172839506172839No Hit
GTAACTGAGAGGTTATGGTCACATCG10.6172839506172839No Hit
ATAAGAGAGCGCCTTGGACCAGGGGA10.6172839506172839No Hit
CTAACTTCATAGAAACTCTTTTTTTA10.6172839506172839No Hit
TGACTAGCACGTCTCTCGCAATTCTA10.6172839506172839No Hit
CATGGCGCGCTTTTCTTATATGGGGT10.6172839506172839No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAA10.6172839506172839No Hit
GGATTACAGTTAACGAAACAAAAAAA10.6172839506172839No Hit
CACACTCTCTTGAGACAAGAAACAGA10.6172839506172839No Hit
GCTCTGTGTGCATCTACCCGACTTGT10.6172839506172839No Hit
GCACTCTAGCCAACAGTGAAAAATTA10.6172839506172839No Hit
CATCAAGGTGAGGGAGATGGGCTTAA10.6172839506172839No Hit
AAGCCGCAGAGTACCGTAAAGTGAGA10.6172839506172839No Hit
CAGAGAGTCCCATTTACAACAACATT10.6172839506172839No Hit
GGAAAGCAGAGAACAGAAAGTCTCGC10.6172839506172839No Hit
GGGAGATCAATCGGTTTGACTCTTAT10.6172839506172839No Hit
TCATTACAGGCCCGTTAGCCAGGGAT10.6172839506172839No Hit
CTCATTATCGTTACGAGATCGATTAC10.6172839506172839No Hit
CAGCTAAAGATGTGTAAGTGTAGTGC10.6172839506172839No Hit
ACGAGCCAGTTACGGGCACAATAACA10.6172839506172839No Hit
GGCGTGTTCACTTGGCTATCTAATTT10.6172839506172839No Hit
GAGGTGACATGCAACTCGGCGTAACC10.6172839506172839No Hit
ATAACTAACAAACCAAAACTAAAAAT10.6172839506172839No Hit
AGGCCACTCCAAATAACATCATCTTA10.6172839506172839No Hit
CTAACTGCACCAACTACACTATACGA10.6172839506172839No Hit
GCACATAAGCTCTCGGATAACACACA10.6172839506172839No Hit
ACGTCAAGTGTTGGGAGCCCTAAAGA10.6172839506172839No Hit
GCAAAAAAAAAAAAAAAAAAAAAAAA10.6172839506172839No Hit
AACTCTTAGAAGGTGATGTTTAAGTA10.6172839506172839No Hit
AGTGTCATATTTGCCTTAGCTCAAGA10.6172839506172839No Hit
CGACTTCAGTTGCAGGTAAGCAGAGC10.6172839506172839No Hit
GATCTAGGTGCGACTTCATTCCGTTA10.6172839506172839No Hit
CTGCCTACACATAACCTTCCAAGCAC10.6172839506172839No Hit
CCTCAGTTCATACGGTCACCAATTAA10.6172839506172839No Hit
AGTGGGAAGTCGAGTGAGTACGTAAG10.6172839506172839No Hit
CTTAACTATCTAAACATTAACAAAAC10.6172839506172839No Hit
TGTCCCAAGTCATCCATAAATATACT10.6172839506172839No Hit
GTCACGGTCTGTCTATCACCTCTGAT10.6172839506172839No Hit
CGACTTCCATCCAACACTACATGTTC10.6172839506172839No Hit
GAACCTATCACAAAAAAAAAAAAAAA10.6172839506172839No Hit
TAGTGGTAGGACGAAAACGAATATAG10.6172839506172839No Hit
CCTCAGTGTTGTCGCGTTGGATCTGT10.6172839506172839No Hit
GAGCAGACATCTGGTAAAACAACACC10.6172839506172839No Hit
CCAATCCGTGTTAAGATTGGCTTCAT10.6172839506172839No Hit
TCTGGAAAGAAACGCCAGCTATAAGC10.6172839506172839No Hit
CTGCCTAGTAGAGTGCTCAGAACCAC10.6172839506172839No Hit
ACTTACTTCTCGTATTAACGATAGTA10.6172839506172839No Hit
TCCAAATAAAAAAAAAAAAAAAAAAA10.6172839506172839No Hit
GAATAAGTCTTAGAGCCTTATCTTGA10.6172839506172839No Hit
TGGACGCCAAGTTGTCCGATCTGAAA10.6172839506172839No Hit
CCACCTATCCAACCAATCTCACGGCC10.6172839506172839No Hit
GGTGAAGTCTACCAGATCCCTATAGA10.6172839506172839No Hit
TGACGGCTCCAAAAAAAAAAAAAAAA10.6172839506172839No Hit
TTGTAGGCAGACGCCTCCAGATACGC10.6172839506172839No Hit
GAAGCAGTCCTCGCATAAGAGCGTTG10.6172839506172839No Hit
TCTGGAATCGTTTAGGGATCCTGTTT10.6172839506172839No Hit
TATCAGGAGCACACAGGCCATGTCAG10.6172839506172839No Hit
GATAAGCAAGATGAGTTCGTCAACCT10.6172839506172839No Hit
AGAGCCCAGCTGCAAGAGAACCCGCG10.6172839506172839No Hit
ACGAACTTGTAACTAAAGTATTTAAA10.6172839506172839No Hit
CCCATACGTTGTACACTGCATATCGC10.6172839506172839No Hit
GCAAACTAGCTGTCTAATTTAGGCGC10.6172839506172839No Hit
CATGACATCTGCTTGCGATGTGAAAT10.6172839506172839No Hit
GGTTGTTCTGGATGTTCCGATCTGGA10.6172839506172839No Hit
AAGTGCTCTCCTCCCTCTCTGGTTTC10.6172839506172839No Hit
ATAACGCGTAATAGCATACATAGTTT10.6172839506172839No Hit
ATAAAAAAGATAAACTCGATCCAATA10.6172839506172839No Hit
GTAACGTTCATTGAGAATAGGTAGAA10.6172839506172839No Hit
AGGCCACCAAGAGTAGTACGATAAGC10.6172839506172839No Hit
GTAACTGAGTATCGAAAAAAAAAAAA10.6172839506172839No Hit
GGATTACCAAGTTGTCTCAAAAAAAA10.6172839506172839No Hit
GATCGTATCCTAGTGATCAACTGAAT10.6172839506172839No Hit
CATCAAGCATTTGCTTTTTATACTCC10.6172839506172839No Hit
AGTGGGACAAGTCTACAACGTCCTAA10.6172839506172839No Hit
TTCAAGCAACACCCGGTGATGGAGCT10.6172839506172839No Hit
GTAACGTGTTAAAGACTCGGCCGAAC10.6172839506172839No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAA10.6172839506172839No Hit
CATGCCTAGAGTGACCCAAAAAAAAA10.6172839506172839No Hit
CATATGGTCCCTAACCATACAAATAT10.6172839506172839No Hit
GGGTCTGGTCCGCTGAACCGCGTTCA10.6172839506172839No Hit
CGGCTAGCACATGACTTATCGGATCA10.6172839506172839No Hit
GAACGGAAGTTGTAGAGTAGGAATTC10.6172839506172839No Hit
TTCTACACATTGGTACATGATAAATA10.6172839506172839No Hit
TGCCAAAAGACTCGTAATTAGAAGTA10.6172839506172839No Hit
GTCAAGTTCCTTTCTCTTTTCCGGCT10.6172839506172839No Hit
AGTCTTTTCGCACTCTATTGCTTGCC10.6172839506172839No Hit
TACCTTAAGTGACTCTACAGACTACG10.6172839506172839No Hit
TCGGGACGTCCAGTATCGCTTTTTGA10.6172839506172839No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers