FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20831493
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7558633.628462923900846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA6700.045.9685331
CGCCGTA15350.044.4321559
CCGTATC16450.041.46737761
GACGTAG7050.041.1980932
CGTATCA16800.041.02031762
TCGCCGT17200.039.85703758
GCCGTAT17350.039.7153860
GATCTCG18650.037.70146647
ACGTAGC7850.037.44011733
TCTCGGT19550.036.6844949
CGTAGCT8300.035.41031634
GACGACG9150.034.8108629
GTAGATC20550.033.87295544
ACGTGTA4300.032.55214340
CTCGGTG22300.032.1606250
TCGGTGG22650.032.1274151
GTATCAT22750.030.44576663
AACGTGT4650.030.10357539
TAGATCT24450.029.7586345
CGAGATA5400.027.22126231
Produced by FastQC (version 0.11.5)