FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194972
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAAGCTCTAAGTGTAGATC263872.208168894333926No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG20200.1690416177115447No Hit
CTCATAGACATAGAGCAAATACATCTTTTTATCACAACCATCTGCATTGA15480.12954278426607485No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC12840.10745021640674425No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA150.002224303370.004944
ACGTTAT207.925977E-570.0049417
CGATTAT150.002224303370.0049422
TTCGACG207.925977E-570.0049418
ATGATCG252.8412833E-669.99908428
CTTATCG353.7143764E-969.99908433
CGCGTAA252.8412833E-669.99908443
TCGACGG252.8412833E-669.99908470
CGCGATC150.002225041969.9990828
CGATTCG207.929263E-569.9990852
TCGGCTA150.002225041969.9990843
TACGATG150.002225041969.9990861
GGACGTA207.929263E-569.9990831
GCGATTC150.002225041969.9990842
ACTCGAC150.002225041969.9990864
TTACGTC150.002225041969.9990828
CGTAAGT207.929263E-569.9990847
CTCGACG207.929263E-569.9990870
TATCGTC207.929263E-569.9990858
TATCGGA150.002225041969.999086
Produced by FastQC (version 0.11.5)