FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906668
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGACGTAGCTTGTGTAGATC952914.997776225331311No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG27030.14176563512892648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA99000.069.1159862
CGCCGTA98300.069.1096959
GATCTCG99850.068.8080347
TGGTCGC99250.068.5187155
GCCGTAT99550.068.2419160
GTAGATC100650.068.19157444
GTATCAT101100.068.09576463
CCGTATC100300.067.8712161
ATCTCGG101300.067.61581448
GTGGTCG100600.067.5992354
TCGGTGG101000.067.5047751
ATCATTA102950.067.1100865
TAGATCT102750.067.1044545
TATCATT102600.067.1002164
TCGCCGT101250.066.95785558
TCATTAA103250.066.9150866
CATTAAA103550.066.7888267
CGGTGGT102350.066.6827852
GTCGCCG101850.066.5977757
TAGCTTG102850.066.5287536
Produced by FastQC (version 0.11.5)