FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10472
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGACCGCATGTGTGTAGATC106110.131779984721161No Hit
ATTCTGTAGCGCTAACAAGAATGTTTTTGTTTCAGAGTCTAGTTGATTCT5174.936974789915967No Hit
CCCTTAGCCTGTGGGTGTGGGGCTGCGCAGGGTGGAAAGCATGGTGCCAG4614.402215431627196No Hit
GAATAGTCAAAGTTATCTCACACCCTTAATCATGTAGGATAAGAATAAAG4504.297173414820474No Hit
CTACCAAGACTAGCTAACTCCTTCCTCTGTGCTGTACTTAATGATAAAAG4464.258976317799847No Hit
ATCCTGCGCTAGTCCCTAAGAACACAGTGAGAAGGACATGAGCAAGCATA4344.144385026737968No Hit
AACCTCAAAAAAGCTGGGTTTAATGTTTCTCAGACTGTTTCCAAGCAGAC4163.972498090145149No Hit
GTGTTAGGAGGAGTGATTGATCCAAATTACCAAGGGGAAACTGGACTGCT3803.6287242169595113No Hit
CTTTAATCCTAGCTTTGCCTTCTCAAATCTTAGAGCCCAGAAGCTGCTTC3763.590527119938885No Hit
ATTCATAACAGTAGCAAAATTACAGTTATGAAGTATGCAATGAAAATAAT3733.561879297173415No Hit
CACATGATCTCTTTACCTATAAATGAAGATAATTTTTATCCTATGTAAAT3723.5523300229182584No Hit
GTGTGTGTGTGTAGACTAGTCAGAGGAAGGTGACTATATTGGGTATCTTT3583.418640183346066No Hit
GTTGTAGACAATAGGTAAGATTTGGAGGAGGCTACACAATGCTCCAGTGT3573.4090909090909087No Hit
ACCCTGACTTCCTTTTCTCATTTGCAAGCAAGTTTGCATGAAGCTTTCTG3403.2467532467532463No Hit
GTGTGTATCTGTGTCTGTCTGTCTGTCTGTCTGCCTGTCTGTGTCTGTCT3012.874331550802139No Hit
CGTTGGAGGTCACAGCAGCTGCCTGCAGAGGGGCGTGCACCAGTGGCTGT2812.683346065699007No Hit
CATCACTGGCAATACTCGCATTCACCGTGTGCTGGAATCCAGGCCCTGTC2802.6737967914438503No Hit
CCCTTAAGGAGGAAACACAAAAATGCCTTAAAGGTTTACAGGAAAACACA2742.6165011459129106No Hit
GGTCAGTCCTGGGGATCCCTTCAGCCTGCAGAGGGTATGAAGAGACAACA2662.5401069518716577No Hit
CCCTGATGCCTTTGACTTCGATCTCATCATTTCCACCCTTAAGAAGCTAA2352.2440794499618026No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACATATGTGTTTTTCAGTG2232.1294881588999237No Hit
GTTCTGTTGCTGGTCCACAGAGTGAGAGACAGGCATGCATGCATGTTTTG1761.680672268907563No Hit
GTCTTGATGGGAGGAACAAAAAGAGATCTCTCTCCTGGAGCCTGTGGAGA1541.4705882352941175No Hit
TAACCATCCTTTGCACAAATACACTATTTATATTTGTTTGAAGGTTTTCT1411.3464476699770818No Hit
GCATTTGAAATGTAAATGAAGAAAATACCTAATTTGGGGGGGAGGGTATG1241.1841100076394193No Hit
CACATCTACTCCAACTCCATGTTGTGCATTACTGTTGTACTTTGCTCATA1071.021772345301757No Hit
ATATGACTCTACATATCGACGAAAAAACCACTTGATTTGACAGGAACACA920.878533231474408No Hit
ACCCAGGACCACCAACGCAGGGATAGTACCACCTGCCAGGGGCTGGGCCC820.783040488922842No Hit
GTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAGTGTAACTCAGTC760.7257448433919022No Hit
GTTTTTACACCTAATGAAATTGCAGTGATTCTTGGGTATTGTCTAATACT620.5920550038197097No Hit
GCAGAGCCAGGTGGATTTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTG470.4488158899923606No Hit
TCTCACTTCTACCCACAGGTCACTGATTTGGTTAGCTAACTTGCTCTATG440.42016806722689076No Hit
GAAGTTCAGTGGGCCACAAGCAGATAATTTGCATTGTGTGACCTTGAGGG420.4010695187165776No Hit
GTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAGTGTAACTCAGTA370.3533231474407945No Hit
GATGTATTGGCTTGCACTCTGTTAGAATCCTGGCACTTAAAGTTAGGGGC340.3246753246753247No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC320.30557677616501144No Hit
GGGTTACTGTTGCTGTGATGTAAGACCGTGACCAAGAACAAGTTGAGGAG280.267379679144385No Hit
GTGTGTATCTGTGTCTGTCTGTCTGTCTGTCTGACTGTCTGTGTCTGTCT270.2578304048892284No Hit
CTTCAACCTCCCTCACAGATCACTGACTAAGGATAGAGGAGGAAGATGGC230.219633307868602No Hit
CTGTCTCTTATAAACATCTGACGCTGCCGACGACCGCATGTGTGTAGATC200.19098548510313218No Hit
AGACAAGGCTGCCCACTTTCCCCCTACCTATTCAACATTGTACTTGAAGT150.14323911382734913No Hit
GTGTGTATCTGTGTCTGTCTGTCTGTCTGTCTGCCTGTCTGTGTCTGTGT140.1336898395721925No Hit
CATCTGTTGGAAGGAACACAGGGTCCCTAAAGAAGGAGCTAGAGAAAGTA110.10504201680672269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGACA309.285395E-870.0000169
TGATGGG309.285395E-870.000015
GGAGATG309.285395E-870.0000146
ACTGTTT750.070.0000134
GATGGGA309.285395E-870.000016
CTTTTCT600.070.0000112
TGGTCTT309.285395E-870.0000169
AGCCCCT150.002163552770.0000166
GATGGCT252.6488578E-670.0000149
AGCCCAG150.002163552770.0000134
TCCTTTT600.070.0000110
TTTGACA150.002163552770.0000136
TTTGAAC150.002163552770.0000170
TTTGAAA150.002163552770.000014
GAGGTCA252.6488578E-670.000016
TTCATTT150.002163552770.0000168
GTAGACT309.285395E-870.0000111
TTGTTGA150.002163552770.0000148
TGTTGAG150.002163552770.0000149
ATGGGAG309.285395E-870.000017
Produced by FastQC (version 0.11.5)