FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19477
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGAGTTAAGGTGTAGATC1599882.13790624839554No Hit
CTGTTGTGATGAAACCACCATCACCACCACCCGTGAGGAAAGTAACTTAA890.45694922215947015No Hit
CACCAGACCTCCACACTCCTTAGCGCCTCACATCTCTCAAGGGGTAGGTG790.405606612928069No Hit
GATAAAGATGGGTAATGAAACATCAAAGCCTGCCTTTACCAGTAATCACA720.36966678646608825No Hit
GAAGAGAAGGGTCACTAAGGACGGCCACAGGTCAGTAGTAAGGTGTTAAG690.35426400369666783No Hit
GAGAGACAGACAGACAGGGAGACAGATTCAGACAGACAGACATACAGATA680.3491297427735277No Hit
ACCCTGGGCTTTCTCTGGGCTCTCTGAGGTCCTGGGCAGGTCGTCTACTC670.34399548185038764No Hit
CTTAACCACGGAGCCAGCCCTCTGGCCCCAACAAAGCATTTTCAACCTTA670.34399548185038764No Hit
CCTCTGGAACTTGCAATTTCTGCCCAATTTCGTTCCTCACTTTCTTCCCT660.3388612209272475No Hit
GGCATTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGA650.3337269600041074No Hit
GAACGGGTTAATCCAAATGGACAGGACGCTCTCGCTTGATCCGCAGGCAG640.3285926990809673No Hit
GAATAGGCTCGCAGGGGTTCTTTTTACTGATTTCACTTATTACTTACTCT640.3285926990809673No Hit
CAGACAGACAGACACAGACCGACAGAGTTAGAGACAGACAGATACAGAGA630.3234584381578272No Hit
CTTCACAGAACACATGAAGAGCCTTACAGAAGTAATCTAAGCAGAAGGAA600.30805565538840685No Hit
CGTAGACTCTTTGTAGGGAAAGGCAGAATTCTTGGTCCCTCGGCAAGAGA600.30805565538840685No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT570.2926528726189865No Hit
CAAAAAGACTAGCAATGTAAGAGTTTATAGACTTTAAGTGAAATAATATG530.27211582892642605No Hit
CCATTACTCTGGTCAGTGGTTGGTGCCCTATCTAGTGTAGATGGACCTTT500.2567130461570057No Hit
AGATGGGTGTGTCAAGTTGATGTTAGAAGTAGAAAAATCTGGGTATTCCT480.24644452431072547No Hit
GCTGTGGGGGTACTGATTAGTTCATATTGTTGTTCCTCCTATAGGGTTGC470.24131026338758535No Hit
CTGTTCCCCTATCCTTATGACCCCTCAAAAATGAAGAAGAGAAAATGTCC430.22077321969502492No Hit
GTCTGTCCTTTGTCTGTCCTGGTCACTCACTGCTTTCTTCCTGTGCAGTT420.2156389587718848No Hit
GGATACATGGGACAATTTCAATATTTTTGTATCTATTGAGGCCTGTTTTG410.21050469784874465No Hit
GACAGATACAGAGAGACAGGGAGAAAGACATAGACAGAGAGAGACAGAGA410.21050469784874465No Hit
CTTAACCACTGTGCCAGCTCTCCAGCCCAGACTACTATAGTCTTAAACAA410.21050469784874465No Hit
GTCTGTCTGTGTCTGTCTGTCAGACTCTCTGTCTCTGTCTGTCTCTAACT410.21050469784874465No Hit
TTTATCACCTTTCCTGTGGGGACCCCGCCCTCTGGTTCCCGTGGGGAAAG400.20537043692560453No Hit
CTCACACTCTGTCAGGACTGCTGGAAGTAGAGAACTGTGATCCTAGTATT390.20023617600246446No Hit
GTATACACCAGTAATTGGAAGCTCACCTTTCGGCGGATCCTGTACTGACA350.17969913230990398No Hit
GATCAGGCTGGCCTCGAACTCAGAAATCCACCTGCTTCTGCCTCCCGAGT350.17969913230990398No Hit
GTTAGAGACAGACAGAGACAGAGAGACAGAGTCAGAGAGACAGACAGAGA330.16943061046362376No Hit
CTCTAACTCTGTCGGTCTGTGTCTGTCTGTCTGACTCTCTGTCTCTCTGT330.16943061046362376No Hit
CTGTCTCTTATAAACATCTGACGCTGCCGACGAGAGTTAAGGTGTAGATC260.13349078400164296No Hit
CTGTCTCTTATACACATCTGACTCTGCCGACGAGAGTTAAGGTGTAGATC250.12835652307850284No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGAGTTAAGGTCTAGATC240.12322226215536274No Hit
GCCGTGGCCCGCGCAGCTCGGGTCTCTGCGTCTGTCTGTCCCCCTGTCCC240.12322226215536274No Hit
CCCTAAACCTAGCCCTAGCCAAAAGCCTAACCCTAACACTAGCCAAAAAC240.12322226215536274No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGAGTTAGGTGTAGATCT240.12322226215536274No Hit
GCATCTACATTCATGATGAACCATCTCTGAAGCTCAGAGCTGAAGAGCTG240.12322226215536274No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGAGTTAAGCTGTAGATC230.11808800123222261No Hit
GTACCTGTGCTTACCAGGGCTCTCATGCATCACATGTCTGTGGGAGGGTA230.11808800123222261No Hit
CTCCCTGTCTGTCTGTCTCTCTGTCACTGTCTCTCTGTCTGTCTCTTTCA220.11295374030908252No Hit
GGATGATGCTCTCCAGGTCCATCCATTTGCCTAGGAATTTCATAAATTCA220.11295374030908252No Hit
GTAAACACACACAGTAAAGTGGGGGGTAAACACACACAGTAAAGTGGGGG220.11295374030908252No Hit
CTGTCTCTATACACATCTGACGCTGCCGACGAGAGTTAAGGTGTAGATCT210.1078194793859424No Hit
GGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCG210.1078194793859424No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCT150.002192075470.0000115
GCCCCAA150.002192075470.0000125
TGTTGTG150.002192075470.000012
CAGCCAG150.002192075470.0000126
ACTGTTA150.002192075470.0000134
CTCTGGC150.002192075470.0000120
ATCCTGG150.002192075470.0000161
CTTCAGA150.002192075470.0000161
ACAAAGG150.002192075470.0000169
ACAAAGC150.002192075470.0000131
TGGTCGA150.002192075470.0000155
GGAGACA150.002192075470.0000118
TGGTCCT150.002192075470.0000144
TCCTCGC150.002192075470.0000147
AAACCAC150.002192075470.0000112
AAGTAAC150.002192075470.0000140
CCAACAG150.002192075470.0000168
CCAACAA150.002192075470.0000128
AACAAAG150.002192075470.0000130
GCCCATG150.002192075470.000016
Produced by FastQC (version 0.11.5)