FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651723
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG13270.20361411213046035No Hit
GAGTTACAGAGACAAAGTTTGGAGCTGAGATGAAAGGATGGACCATGTAG7440.11415892948384514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG252.8385948E-670.004237
GTCTACG252.8385948E-670.004232
AGGCGCG252.8385948E-670.0042319
AGACGAC252.8385948E-670.0042316
CGCTATA252.8385948E-670.0042315
GTGTCGT500.070.004236
CGGCTAA252.8385948E-670.0042322
TACTACG252.8385948E-670.004235
ACCGTCC500.070.0042322
CGTCCGT252.8385948E-670.0042320
TTCGAAC252.8385948E-670.0042317
AACCGAC353.7089194E-970.004222
CTCACGG401.3460522E-1070.004227
GTCGCAC550.070.004221
TACCCGA207.9236765E-570.0042221
GGAGCGC207.9236765E-570.004226
CTATCCG207.9236765E-570.0042220
GACGTGT207.9236765E-570.004227
ACCGACC353.7089194E-970.004223
CGCGATC207.9236765E-570.004222
Produced by FastQC (version 0.11.5)