FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770961
Sequences flagged as poor quality0
Sequence length76
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCGA207.927544E-569.9989647
ATCGACG252.8403556E-669.9989623
ACGATAC308.375002E-658.3362543
TTCGCGA252.389951E-456.002815
TCGTTAT252.390717E-455.9991757
CGATATA452.7022907E-854.44363439
TACGGTC200.006949797352.502635
CGTTATG200.006951580352.4992211
ATAAACG200.006951580352.4992241
GTACGAT506.198752E-848.9992736
GCACGAA604.9385562E-946.66597712
TTCGTTA305.878623E-446.66597756
TAACGAG305.878623E-446.66597754
ATATCCG305.878623E-446.66597753
TTACGGT305.878623E-446.66597710
TAACCGT404.592035E-543.752196
CGTACGA404.5937933E-543.7493542
GGCGTAA503.4797467E-641.9993748
ACGATAT602.5986446E-740.8327338
TACGATA602.5986446E-740.8327337
Produced by FastQC (version 0.11.5)