FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230021
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG150.00222499869.9995641
TACTTCG252.8412196E-669.9995668
TAGCGAC301.02440026E-769.9995637
TAATCCG252.8412196E-669.9995655
GCGACCC207.929087E-569.9995523
TCGGACG207.929087E-569.9995555
TAGATCG207.929087E-569.999555
GCGACTA352.976285E-759.9996239
ACCGCTA352.976285E-759.999625
CGTCCGT352.976285E-759.9996230
CGTAAAG308.380757E-658.33296268
TCGACGT308.380757E-658.33296238
CTTAGCG308.380757E-658.33296261
TATCGGT308.380757E-658.33296238
CGTATAC1150.057.8280683
GCTAGCG553.6379788E-1157.2723646
GGTACGG750.056.0019152
CGATCTA252.3911786E-455.9996455
GCGCTAG452.7039277E-854.444123
CGATACT200.0069512152.501817
Produced by FastQC (version 0.11.5)