FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869040
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC350.001256062539.99940514
CGATTAA708.70481E-734.99947729
TTCGTCA7400.031.21575244
GTAGGAC16200.031.1131463
CGTACGT450.00430776831.11314422
TGACGAC7200.029.65233661
TCGTCAA6300.029.44400460
CGATTCG604.985908E-429.16623314
TTTCGTC7600.029.01272643
TTGACGA7400.028.85092260
TCGTCAT7700.028.18139845
CGTCATT7600.028.09168846
GACGACT7500.027.99958462
AATCACG8250.027.99958234
GATCGTA500.00721203227.9995855
TGTAGGA18100.027.8471242
CGTCAAG6300.027.77736361
GTCCTAC13750.027.4941771
TATACTG8150.027.4849875
TAGGACC10450.027.4659124
Produced by FastQC (version 0.11.5)