FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898496
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTACCAACCCTGACTGCCGATCAGAAAAAACAGTTTGGTCCTTTTG11270.12543183275162048No Hit
GATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGG10780.11997827480589786No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGGA10320.11485860816297458No Hit
GGCTAGATGTTGCTAAAATAAATAAAATCCCTAGGTTTAAATTAATTAAT9740.10840337630885391No Hit
CCATAAAGCCGCCCAGACGCGCAATCGCCATATACGCCCATTGCAGGCTG9020.10038998504166964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATG353.7107384E-970.0032922
GTCCGTA800.070.003292
GCGTCCC401.3460522E-1070.003294
AGTCGAG207.9258345E-570.0032921
ACTCTCG650.070.0032816
CGTCCCA150.002224331570.0032822
GTACCTA353.7125574E-969.999398
TCGCACG800.069.9993966
TTAGCAC650.069.999395
CCCTAGA600.069.9993928
TCCGAAG353.7125574E-969.9993924
TAGGTAC353.7125574E-969.9993935
CGCATGT800.069.9993937
GTCTCGC353.7125574E-969.9993932
CGTCTTA353.7125574E-969.9993923
CTAAGTA600.069.9993912
AAGCGTC353.7125574E-969.999397
ATTCCGT550.069.999396
CTCGCTA800.069.9993956
GACGGCC301.024091E-769.9993950
Produced by FastQC (version 0.11.5)