FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442549
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA601.2173838E-534.99977561
TATACTG13950.025.3406245
CGTCGTG2100.025.00096521
AATCGAC2100.024.9998442
GTAGGAC26900.024.3312453
GTCCTAC31850.023.956431
CGTAACG750.00183392223.33318369
TATACCG2600.022.8847265
GCGACGT1854.0563464E-1022.70255763
CTATACT14600.022.5346094
TCGTTCG1403.2901153E-722.50086822
CGATCGT1102.9260396E-522.27258523
CGATACG1102.9260396E-522.27258526
GTATAGC12200.021.8036291
TTCGTAG2259.094947E-1221.77763710
TCGTACC2001.0713848E-921.000342
CGACGTA1003.9362482E-420.99986550
TAGTCGA1003.9362482E-420.99986567
TCCTACA38200.020.890392
GTCTACG4900.020.7146171
Produced by FastQC (version 0.11.5)