FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704112
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23710.33673620105892244No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT11010.15636716885949964No Hit
GTCCTACAGCTGCTTCGCAGGCTGCGAAAACTAAGATGGTGATGGGGATT10710.15210648305951324No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT10570.15011816301951963No Hit
ATCCAACATTTCATCATGATGAAACTTTGGGTCCCTTCTAGGAGTCTGCC9500.13492171699956826No Hit
CCATAACACAGTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACA9100.12924080259958642No Hit
GTGTTGATACAGGCGCTGTATGCCTCTTTCAGACGGCTGTCTTCCTGTGG9020.12810461971959006No Hit
ATTTACAGCTATTTGTGCTCTCACCCAAAACGACATCAAAAAAATCATTG8950.12711045969959325No Hit
CCCTAGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGA8930.12682641397959415No Hit
GACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGG8700.12355988819960462No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA8650.12284977389960688No Hit
GTCAGGTGGAAACCTGTGACCAGAATAGAAGTGAGTTAGTAACACTACCC8110.11518053945963143No Hit
CCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGC8090.11489649373963233No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA7950.1129081736996387No Hit
GTATTATCCTTATTTATTATAACTTCAGTAACTTCCCTAAACTCCAACTC7830.11120389937964414No Hit
GTTTTAGATAGTTCCGCAAACCTTCTCAATCAATTCCGCCGGGAATTGCA7750.11006771649964778No Hit
AGCGAAAAGACTATAGAGTGAAGGAGAAATTTTGAGGGTGCGTCTCACCG7740.10992569363964823No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA7290.1035346649396687No Hit
ATGCTACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACT7140.10140432203967552No Hit
GGCGGCAGGTAAAGGCGGGATTGGACTTGCAGCAACACGTCTTGTTTATT7140.10140432203967552No Hit
GGCTCATACAATAAAGCCTAGAAAGCCAATAGACATTATTGCTCATACTA7050.1001261162996796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAG455.456968E-1270.0000122
AGACTCG550.070.0000125
CAGTTAG455.456968E-1270.0000158
CGTCTTA455.456968E-1270.000016
CGCGAGT550.070.0000166
GTTACTA550.070.000012
CACTCGA455.456968E-1270.0000155
TATTCGA455.456968E-1270.0000167
GGTGCGA550.070.0000150
TACTCGT455.456968E-1270.0000118
CGACTCA455.456968E-1270.0000160
TATAACG550.070.000014
CTAGGGG455.456968E-1270.0000132
ACGGTCC455.456968E-1270.0000149
GTTGACG252.83985E-670.058
GTGCAAC252.83985E-670.045
GAGTGCG150.002224545570.07
TAGAGCA150.002224545570.042
GTCGCTC252.83985E-670.08
TCGTTAG600.070.03
Produced by FastQC (version 0.11.5)