FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5075919
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66160.13034092939623346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC34250.028.4098471
TATACTG16850.025.9648725
GTAGGAC30850.024.7332823
TATGGCG24900.024.03753716
CTATACT19850.023.0988924
CTACAGT42650.022.8963154
TCCTACA45250.022.8183292
CCTATAC16450.022.7666053
TTCGTCA16600.022.1383144
CGTCATT16250.021.75360746
TACACTG32350.021.7469275
TCGTCAA14800.021.52003560
ATTTCGT17200.021.1625642
GTACCGT3000.021.0003916
GATTTCG17000.020.9997741
TTTCGTC17350.020.97959743
CACGTTT18000.020.61088628
TCACGTT17500.020.59977327
TACAGTG48700.020.4829255
CCTACAC28150.020.142673
Produced by FastQC (version 0.11.5)