FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5980947
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGA353.7180143E-969.9993643
TCGTACG800.048.12456554
TTCGACG459.207033E-538.88853569
CGTACCG1850.035.94561813
CGTCGGA400.002420350734.99968365
GTCCTAC59450.033.7942121
TCTCGCG851.06636435E-732.94087647
CGGTCGT652.1056218E-532.307447
CGTTCGA1206.548362E-1132.08304258
CCGCGTC1001.26219675E-831.49997523
CGTCGCG802.4710716E-630.62472348
AATCACG25800.030.11600734
TCCGACG952.8279828E-729.47366324
TCGCGAC1551.8189894E-1229.3562920
TGGCGAG55800.029.35628918
CGCGACA1202.3046596E-929.16810821
TCGGACG853.962936E-628.82326735
TACGTCG853.962936E-628.82326736
GGCGAGA32600.028.77442719
GCGAGAA32750.028.74951420
Produced by FastQC (version 0.11.5)