FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5487498
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCG404.59727E-543.74970245
TCGCGTA503.483463E-641.99971447
GTCCTAC69200.032.977861
CGACGTA1109.094947E-1031.8179669
CCGTGAT25100.029.1432327
CGTGATT24950.028.61703928
AATCACG32050.028.50214834
ATCACGG32550.028.38690435
TCGCGAC500.00721133928.00108516
ATCGTCG755.5921184E-527.9998164
TATGGCG60550.027.97796616
TCCTACA83500.027.4137362
GTCGCGC906.1814535E-627.22203849
ACGGAAA36650.027.21673238
TCACGGA33850.027.19331636
TCCGTGA27300.027.051126
CGGAAAA37250.026.9662639
GTAGGAC77550.026.7187963
ATGGCGA64400.026.63146817
CTACAGT90650.026.5256424
Produced by FastQC (version 0.11.5)