FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5685682
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC56700.030.9897651
TATGGCG48200.029.19260216
TCGACGG500.00721125728.0011674
TTTCGCG755.5923156E-527.99969125
ACGCCCG2800.027.5016321
TGGCGAG51750.027.25765618
GCGAGAA31600.026.91615320
CGAGAAA32800.026.78512421
ATGGCGA54550.026.75685717
GGCGAGA31600.026.6946219
TAGGACC37650.026.5880954
GTAGGAC63950.026.5452823
CCGTGAT21600.026.41174527
ACGTCGG808.677232E-526.2497161
TCCGTGA22550.026.23030926
AATCACG26550.026.10140834
TCGTCAA23800.025.7350160
TTCTCGC23150.025.7016642
CGGAAAA29900.025.51811239
ATCACGG26450.025.27382335
Produced by FastQC (version 0.11.5)