FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241152
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC38000.031.6848321
CCGTCGA450.00430984631.1107670
TCGACCG450.00430984631.1107663
AATCACG16450.029.99966434
CGAACGT604.986148E-429.1667928
CGTCATT16450.029.1486146
TATGGCG31350.028.8052816
TCACGTT18650.028.33792127
TTCGTCA17700.028.0787844
CGACGTA500.0072126727.99968755
CGCGATA500.0072126727.99968710
GCGAGAA19700.027.71713420
ACGTTTT19200.027.7084529
CACGTTT19200.027.7084528
GTAGGAC41000.027.6590793
ATGGCGA34500.027.39268717
AAGTCGT17300.027.31183257
TCGTCAA17700.027.28782860
ATCACGG17600.027.24401335
TCGTCAT17650.027.16683645
Produced by FastQC (version 0.11.5)