FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8698143
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAAGTGATTCGTGTAGATC208950.24022368912536848No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181230.20835481780421405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA36150.044.1491862
TCGTGTA38150.041.92642240
TCTCGGT38400.041.4711749
CGCCGTA37950.041.0406659
ATCTCGG38600.040.98427248
GATCTCG38650.040.47847747
CGTGTAG39000.039.93572241
TTCGTGT38750.039.92240539
GCCGTAT39250.039.68135560
CTCGGTG40100.039.36391450
CCGTATC40250.038.78243661
CTAGACG13600.037.573583
TGGTCGC43500.037.33316855
GTAGATC42350.037.02462844
GTGGTCG44900.035.23369654
GGTCGCC46550.034.661556
TAGATCT45700.034.61691745
TCGGTGG46500.033.7202851
GTCGCCG48200.033.692857
TCGCCGT48450.033.446758