FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15269868
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC80950.024.2128451
TATGGCG47800.022.69983116
TATACTG68750.022.6037585
GTAGGAC85650.022.3937283
CTATACT66350.022.1555024
TCGTCAA29200.021.69503860
GATTTCG31800.021.24197441
AATCACG31650.021.12147734
TACACTG119950.020.862975
CTACACT87000.020.0749514
CCTATAC57800.019.619613
ATCACGG33300.019.54939735
CGTCAAG32050.019.5474361
CGTCATT33050.019.48547646
TTCGTCA33450.019.357144
AAGTCGT30350.019.14317957
AGTCGTC30450.018.9653758
ATGGCGA60000.018.95924217
ATTTCGT35850.018.74462542
GCGAGAA39650.018.71464220
Produced by FastQC (version 0.11.5)