FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19933849
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA30200.047.40020862
GCCGTAT33200.042.5899560
CGCCGTA34050.041.52676859
CCGTATC36700.038.90970661
TCGCCGT42800.033.36416658
CGTGTAG46850.031.2270241
GTGGTCG48850.029.37535754
CCGTGTA50350.028.84778840
TGGTCGC51700.028.16220755
GTATCAT56400.026.00152263
TCTCGGT56350.025.90037249
ATCTCGG56400.025.75329848
GATCTCG58250.024.93538347
GTCGCCG59550.024.50858157
ATCATTA65950.022.60781965
ACCGTGT64650.022.46691539
CTATACT63700.022.14324
TCGGTGG67000.021.9400951
GTAGATC67550.021.81326144
TATACTG63700.021.8130325
Produced by FastQC (version 0.11.5)