FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n02_dpr167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n02_dpr167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5817306
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG5650.032.83251
TAGACGC13700.032.7013664
CTAGACG8650.031.5613123
GTCCGCA8650.031.56049732
TAGACCG6850.030.6575324
TCCGCAA9300.030.48368333
CCGCTAG1052.0296284E-829.99981514
CCTACGG9150.029.8358812
CTACGGT10100.027.02953513
TAGGGCG11450.026.89939531
CGTACAC6400.026.2505113
GACGCAC10700.025.8411836
TTAGACG6400.024.062973
TCGGACG3700.023.64849955
ACTATAC29900.023.6459473
ACCTACG11850.023.0378311
ATTCGAA6250.022.95985432
GTATTAG26850.022.942721
CGAATTG6450.022.79153422
CCGTGAT12450.022.7709427
Produced by FastQC (version 0.11.5)