HGLJYAFX2_n01_undetermined.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HGLJYAFX2_n01_undetermined.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20831493
Sequences flagged as poor quality	0
Sequence length	76
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	297348	1.4273964904963845	No Hit
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	283248	1.359710511387734	RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG	27740	0.133163763154182	RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG	22965	0.11024173831419573	RNA PCR Primer, Index 16 (96% over 28bp)
GGACAGCCGAGCCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG	22370	0.10738548600429168	RNA PCR Primer, Index 16 (96% over 28bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCTTGAA	48780	64.764145	34
CTTGAAA	49860	63.7596	35
ATGCCGT	52045	62.945812	22
TTGAAAA	50800	62.926746	36
CTCGTAT	51555	62.866344	17
TCTCGTA	51020	62.864544	16
ATCTCGT	51275	62.59028	15
AGCCCAT	53460	61.054417	10
CAGCCGA	54175	60.973534	4
AGCCGAG	54310	60.905685	5
ACAGCCG	54415	60.68547	3
GGACAGC	55680	59.52577	1
GACAGCC	55820	59.09833	2
AAGGGGG	37410	54.732277	47
AAAGGGG	37455	54.535103	46
AAAAGGG	37590	54.45375	45
CGAGCCC	60845	54.25901	8
GAGCCCA	61240	53.937595	9
CCGAGCC	61395	53.767246	7
AGGGGGG	38925	53.48283	48