FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194972
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGA270712.2654087292421914No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC27940.23381300984458211No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG26110.21849884348754617No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAA150.002224366670.0044439
CGGTATA150.002224366670.0044441
GGCGAAT150.002224366670.0044437
GGTACGA252.8406994E-670.0015145
CGAACAT150.002224735770.0015169
GTAATCG150.002224735770.0015146
TATTACG301.0241638E-770.0015136
TTCGCTA252.8406994E-670.0015135
TAATCCG150.002224735770.0015169
ATTCGGA207.9278994E-570.001544
TCGACTA353.7125574E-970.001534
CGATAGC207.9278994E-570.001517
CGAACGA150.00222510569.9985853
GCGAATA150.00222510569.9985811
TACGGAC301.024473E-769.9985852
ATCGTAG150.00222510569.9985813
CCGTATA150.00222510569.9985852
TCGCTAC150.00222510569.9985828
ATGACGG150.00222510569.9985863
TATACGA301.024473E-769.9985852
Produced by FastQC (version 0.11.5)