FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906668
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGA965265.062548907308456No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG36180.1897551120593622No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC35110.18414322787186863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG91300.068.2348565
CTCGTAT90000.068.0149364
CGTATGC94100.067.7683866
TCTCGTA84450.067.221563
ACAGCCG101150.067.0917150
TATGCCG95350.066.8432668
CAGCCGA101700.066.763351
AGCCGAG101950.066.6682452
CATCTCG86050.066.5410161
ATGCCGT94700.066.4150569
ATCTCGT85950.066.2519562
TGCCGTC87100.065.82094670
GTATGCC97400.065.5082667
CCGTGTA104700.065.4237336
CGTGTAG104600.065.3206837
ACACCGT106000.065.1115433
ACCGTGT105750.065.0372235
GCCGAGC104450.064.97201553
GTAGACA106400.064.2156340
GTGTAGA106850.064.0434638
Produced by FastQC (version 0.11.5)