FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10472
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGA113010.790679908326966No Hit
GAGCCTTCCTCTTTACTGCTTTATTGGATGGAGGACTAGAGGCAGTAGAA5214.975171886936593No Hit
GGAGACAGCAGAGCCAGGGTCAGCCTCCAACCCAAGCCACAGACTCTGCT4724.507257448433919No Hit
CGCTAAACTAGTGAACTTTTGTGTTTGATAACTGACCATCTAGCAAATGT4614.402215431627196No Hit
GGCTGTGGTCATGCTAAGGGACTGGCATCAATTATGTAACAACTGGGAAA4484.27807486631016No Hit
GATGTGCTATGTATGGGCTTGCGAGGTATGCAGATTTTCTCAGCATATGC4414.211229946524064No Hit
GTGGATATGAGTTTGTTAGTATTTCAGTCTGCTTGGAAACAGTCTGAGAA4163.972498090145149No Hit
TTCACGTACAGATGACCTTGCCCCCAGCGGGAGGAAAGACAGTTTTCGCC3943.7624140565317035No Hit
GTCTGAATTAGGCTGTTGTAGTTTCTTGTTGATTTTAATCACAGGACATG3903.724216959511077No Hit
CTGTAGCTTTGTGGTCTGTTTTAAAATCAGAAAGGATGCTCCTAGTTTTG3763.590527119938885No Hit
CTTGCTACCTTAATCTTCAACGCGTCTTCTTGTGGATCCCTACCATGGAG3643.4759358288770055No Hit
GTATGTACCTGCGAAGCAGTTTTATCTGACCTGGTGGGCTAGCGCTGCTC3583.418640183346066No Hit
GTACCAGGACAGTCAGGGCTACACAGAGAAACCCTGTCTCAATGCCCCCA3523.361344537815126No Hit
GACACAGACACAGACAGGCAGACAGACAGACAGACACATACACAAAGAGA3042.9029793735676086No Hit
CCACTGGTGCACGCCCCTCTGCAGGCAGCTGCTGTGACCTCCAACGCTGT2972.8361344537815127No Hit
GGCCTGGATTCCAGCACACGGTGAATGCGAGTATTGCCAGTGATGCTGTC2872.740641711229946No Hit
TCCTAATGCTGTGACCCTTTAATACAGTTCCTCATGTTGTGGTGATCCCC2802.6737967914438503No Hit
CATCTATGGCTCCTGACTTCTTTCCTAGGTTTTCTATCTCCAGAATTTTC2802.6737967914438503No Hit
GGCCCAGTGGTGACATTTCAGTCACCAGCTCTGCTGATATAAATAAGATC2782.654698242933537No Hit
CCATGGGCCTACCCAGTCCTTTTTCCGGCTATGGGTAGTGAAATCATAGA2442.3300229182582126No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA2252.148586707410237No Hit
GTTTTTTCCCACTCATGGGTGCCTGTTCCACTGTCTGCCCTGCTATTTGT1771.6902215431627197No Hit
GTGGGGCACCTGTCCTAGCTGAGAAGCCGGGCCACCCTTTGCCTGTCTCT1541.4705882352941175No Hit
GGTTCAAAGAGATAACCCAAATGGGGTGCCTCAGAAAAACCTGGCTATTC1461.3941940412528648No Hit
GAGTTACACTGAAAAACACATATGTTGGAAACCGGCATTGTAGAACAGTG1261.2032085561497325No Hit
CAGGGTAGGTCTCATGATAGGTTATAGAGATGATTAAAGGAATATACATA1101.050420168067227No Hit
GATGAAAATCGCTCCTGAAATGAAAAAAGAAATTTTTTGAATGTGTTCCT940.8976317799847212No Hit
TTCAAATGCTATCCCAAAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG900.8594346829640948No Hit
CACCATTTGAGCTGCCAGAAAAGGCCTCATCTGAGGGCAGCACCCATTCC870.8307868601986248No Hit
TATAAAGACTTTGGTTTCAGCAAAAGAGGTATGACTTACATTGACTTGCC650.6207028265851795No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG600.5729564553093965No Hit
GCTGCTTGCTGCTTCTGCTCCCTCTACCCTCATGGGCCTCAAGGAAAAGG460.439266615737204No Hit
TCCTAATGCTGTGACCCTTTAATACAGTTCCTCATGTTGTGGTGATCGCC450.4297173414820474No Hit
GACAGACAGACAGACAGATAAACAAACAAAAAAAACCTCTCAAGGATCAG370.3533231474407945No Hit
ATTCAAGGCTGACAGTTTGACGGCATAGAAATCAGCTTTTAAAAGTGATC340.3246753246753247No Hit
GTGGAGCAAAGTTACTGCAGAATGACACCTGAATAGTTGGATGATTTTAA340.3246753246753247No Hit
CCCTTCAAGCAAGAAAGGGTTTATGTAAATTTTATTGTATAGGAGTTCTC330.31512605042016806No Hit
GACACAGACACAGACAGGCAGACAGACAGACAGACACAGACACAAAGAGA320.30557677616501144No Hit
TCCTAATGCTGTGACCCTTTAATACAGTTCCTCATGTTGTGGTGATGCCC260.2482811306340718No Hit
TTCAAATGCTATCCCAAACGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250.2387318563789152No Hit
GGTCTGCATCCCCTTGTTCTATTAGCCATCTTCCTCCTCTATCCTTAGTC230.219633307868602No Hit
GACACAGACACAGACAGGCAGACAGACAGACAGACACATACACAAACAGA200.19098548510313218No Hit
GTTCAAGGCTGACAGTTTGACGGCATAGAAATCAGCTTTTAAAAGTGATC190.18143621084797554No Hit
TCCTAATGCTGTGACCCTTTAATACAGTTCCTCATGTTGTGGTGATGGCC140.1336898395721925No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGGTAGGGTTAGG130.1241405653170359No Hit
AGACACAGTTCTATGGGGTACTGGTTAGTTCATATTGTTGATCCTCCTAT110.10504201680672269No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCG150.002163552770.0000161
TGATGCT252.6488578E-670.0000141
TGGTTTT150.002163552770.0000162
CTACACA309.285395E-870.0000119
GTATGTA600.070.000011
CTCTGCA252.6488578E-670.0000117
CGCTGCT600.070.0000143
AACGCTG252.6488578E-670.0000143
ATCCCAA150.002163552770.0000111
ACAGTCA309.285395E-870.000019
AGCCCAG150.002163552770.0000157
TTTGAAT150.002163552770.0000136
TAGCGCT600.070.0000140
TGTTGGA150.002163552770.0000123
TGTGAAA600.070.0000159
TTTTGAA150.002163552770.0000135
ACACGGT252.6488578E-670.0000116
ACCGGCA150.002163552770.0000131
AATCTTT309.285395E-870.0000152
AAACCGG150.002163552770.0000129
Produced by FastQC (version 0.11.5)