FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19477
Sequences flagged as poor quality0
Sequence length76
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGA1551179.6375211788263No Hit
GCCATGGGTACAGTGTGCTCGGCTGCTGGGGCTCCTGCTGCTGGGCCTTC910.4672177440057504No Hit
GTAGTGGACTGTCTGGTTTGTCTTCAGTGAGAGAATGTGCACCTACCCCT790.405606612928069No Hit
GAAAAATACCTACCTAATTCATTCCCATTGATGTTTCAAGTTCATCGATA710.3645325255429481No Hit
CATTGGAGCAGGTGACAAGTAGGTCACCATGGAGGTCACAGCTCAGTGGG700.35939826461980795No Hit
GAGTGACCCTGTTCCATAGAGCATAAGTCATTGCACAAGCTACCTTTGTG700.35939826461980795No Hit
CTCATAGGTTACCAGTGTCTTGGGCACTACTGGAACCTGGGCAGGGCATC700.35939826461980795No Hit
AACCTGGACTGTTGAGCAGTTAGAGCAGTAACTATGATTCTTGTGAGAGT680.3491297427735277No Hit
AATCAGAACAGATGCTGGTAGAGAATTGGGTCCCCTCCTCCAGCGGGATC670.34399548185038764No Hit
CTCGATATCTGTATGTCTGTCTGTCTGAATCTGTCTCCCTGTCTGTCTGT670.34399548185038764No Hit
GATGGATCTGTTTGTTGAACCCTTTGTGGAGAATTCAAAGACTCATTTGG670.34399548185038764No Hit
ACGGTTCAGGGATACAAGCCACATTGCCATATTGGGGATGACGAATAACT650.3337269600041074No Hit
CGGCTAAGTACCGCGGTTCTTACCTAGGTAAAATGAGCTCACAAAAACAG640.3285926990809673No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACAGTGTAGACAGGGA620.3183241772346871No Hit
CTGTCTCTTATACACATCTCCGAGCCAACGAGACACCGTGTAGACAGGGA610.31318991631154697No Hit
GTGTCTGTCTGTCTGACTCTCTGTCTGTCTATGTCTTTTTCCCTGTCTCT600.30805565538840685No Hit
CCATATGACTCACTTCAAGACACATACTTGCCTTAAAAGAAACTGAACCA600.30805565538840685No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC570.2926528726189865No Hit
ACTATTATGTGCCCAATAGTTGTTCAAAACACATGGTTGATCTCAATTTC540.2772500898495661No Hit
CTGTCTCTTATACACATCTCCGAGCCCACAAGACACCGTGTAGACAGGGA530.27211582892642605No Hit
GGTCCTGGCTCAGCCAGGAGACAACAGCTGCCTTTTAGCCTCAGAGTCCC490.2515787852338656No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGAAAGGGA490.2515787852338656No Hit
GCATGAAAGAATCATCTCTCTGCCACATTTTAATGAGGAATACCCAGATT470.24131026338758535No Hit
CAGAGAGACAGTCCGAGAGAGACAGAGACAGAGAGACAAACAGACAGACA470.24131026338758535No Hit
CAAACAGACAGATAGACAGGGAGTCAGACAGACAGACACAGAGAGACAGA470.24131026338758535No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAAACACCGTGTAGACAGGGA450.2310417415413051No Hit
CCCCTAGCCCCACCAAAAGAAACAGCTTAGACAAGAAGCTATTGAAGAGA450.2310417415413051No Hit
GATATAGCTGTCTCTTGTGAGGCTATGCCAATGCCTGGCAAATACAGAAG450.2310417415413051No Hit
ACAGGGACACAGTGAAACTAACAGAAGTTATGAAACAAATGGACCTGACA430.22077321969502492No Hit
CACACATACACACATGCCACATGAAGACATACATATACCACTTCCATACA420.2156389587718848No Hit
CTGTCTGTCTGTCTCGCTGTCCCTTTGTCTGTCTCTCTGTCTCTGTCTCT420.2156389587718848No Hit
CCCGTATTCCTGTAAGGAAGTAACCCCAATAAAACTCATTAGTGCACCAT410.21050469784874465No Hit
GAGTCGGGAAGAGCGGGAGCACAGGCGAGGGGAGGAGCGGGCTGGACTCT400.20537043692560453No Hit
CGGCTTCCCCGGCCGAACCCCCTTGACTGCGCGCCGTGCCGAGGGCAGAT380.19510191507932434No Hit
CTCTGTCTCTGTCTCTAACTCTGTCTCTCTGTGTCTGTCTGTCTGACTCC380.19510191507932434No Hit
CCACTACCTGAACGAGCTTGAACTGTATCGTGGAATGTCAGTACAGGATC360.18483339323304412No Hit
CTGTCTCTTATACACATCTCCGAGCCCACCGAGACACCGTGTAGACAGGG360.18483339323304412No Hit
GACAGCGAGAGACAGAGACAGAGGGACAGACAAAGAGACAGAGGGACAGA340.17456487138676385No Hit
GGGTCTCTCTACATAGCCTTGGCTGTCCTGGGACTCACTCTGTAGACCAG340.17456487138676385No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGCCACCGTGTAGACAGGGA330.16943061046362376No Hit
GTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGA320.16429634954048364No Hit
GGCCTAGAGGACACGATGGGCCCGGGTTCCAGGCCGATGTGGCCCGGTCA300.15402782769420342No Hit
GGGCGTGGTCTTAGACAGGAAGGGGTCTCATCTCGCACTTTGGGGGCCTT300.15402782769420342No Hit
GGAGAAGGGAGAACCGGGACAGGGGGACAGACAGACACAGAGACCCGAGC300.15402782769420342No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCCTGTAGACAGGGA280.14375930584792318No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGC250.12835652307850284No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGCAGGGAC230.11808800123222261No Hit
ATGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAGACAGGGA230.11808800123222261No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCGTGTAAACAGGGA230.11808800123222261No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAACGTGTAGACAGGGA230.11808800123222261No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACATCGTGTAGACAGGGA230.11808800123222261No Hit
GAAGGATCATCTTTGCACACTCACTTTTCACAAGAGGAACAGTACCCTCC220.11295374030908252No Hit
GAATTTGAATGAGTAGGCACAAAAGTAAATAACATTTTGTCAGCCCAGGA210.1078194793859424No Hit
GTTCATAGCAGCCTTATTTATAATAGCCAGAAGCTGGAAAGAACCCAGAT210.1078194793859424No Hit
GCCACGCACCTCGGGAGAGGCGATGGGGGGGTGGAGCGAGGCCCCGCGGG210.1078194793859424No Hit
CCCCCTCTCTCCCCCTCCCCTTCAGCACCCTGGGCCTCCTGTGTCTGAGA200.10268521846280226No Hit
GGGTTGGGGTTAAGGGTTAGGTTAGCGTTAGGGTTAGGTTTTTGGCCAGG200.10268521846280226No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGG150.002192075470.0000158
ATGGGTA150.002192075470.000014
CAGCTCG1200.070.0000161
GATGGGC150.002192075470.0000159
AGCCCCT252.7373353E-670.0000157
GTATGTC150.002192075470.0000111
ACAGCAG150.002192075470.0000150
AGCCTAC150.002192075470.0000168
ATGGGCT150.002192075470.0000160
AACGTGT150.002192075470.0000135
TCCTCAT150.002192075470.0000149
CTGAGTC150.002192075470.0000119
TGCACAA150.002192075470.0000132
GTCATTG150.002192075470.0000127
CCGGAGC150.002192075470.000019
CGATATC150.002192075470.000013
GTTTGAT150.002192075470.0000161
AATCTGT150.002192075470.0000128
GTGTTCC150.002192075470.0000148
CATTGCA150.002192075470.0000129
Produced by FastQC (version 0.11.5)