FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651723
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC16730.25670415191730234No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG15160.23261416276546937No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA8370.12842879566932577No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA7180.11016950452876452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG353.7089194E-970.0048437
GTAACGA353.7089194E-970.0048441
CGGAATT207.923335E-570.0048338
GTTCTCG252.8384475E-670.0048341
CGGCCGG301.0231088E-770.0048343
GACGTAC150.002223861970.0048342
GACCGAA207.923335E-570.0048337
TTCGTTA301.0231088E-770.0048342
AAGCGTA252.8384475E-670.0048341
AAACGGA301.0231088E-770.0048338
AACGGAT207.923335E-570.0048341
TACGCGG301.0231088E-770.0048339
TACGCGC301.0231088E-770.0048343
AATTGCG207.923335E-570.0048341
TACGAAG500.070.0048343
TTGCGTA207.923335E-570.0048343
CTACCGA207.923335E-570.0048337
CTCGCAA252.8384475E-670.0048338
ACTCGAT207.923335E-570.0048341
TACTCGA207.923335E-570.0048340
Produced by FastQC (version 0.11.5)