FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770961
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATA207.927253E-569.9994866
GATCGAC352.9748298E-759.99955750
TCGATAC252.390629E-455.99958867
CCGTTAT452.7008355E-854.447571
GATATCG200.006951376352.49961557
CGTAATT200.006951376352.49961528
GCGTCAT200.006951376352.49961555
TAATCGC200.006951376352.49961548
CGTAACA900.050.5551822
ACGATCG352.0869235E-549.9996326
ACGACGT352.0869235E-549.9996368
CCGTCGA305.878407E-446.6663228
TAACCGG305.878407E-446.666325
CGTAGAT305.878407E-446.6663269
ACGTCGG305.878407E-446.6663252
ACCGTTA305.878407E-446.6663246
ATCGGCA305.878407E-446.6663213
TAGTGCG305.878407E-446.666329
TCGACTA305.878407E-446.6663221
CGATTAA451.6851518E-646.66631758
Produced by FastQC (version 0.11.5)