FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230021
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA150.002224941570.067
CGTCCGT252.8411123E-670.061
ATTCGAG501.4551915E-1162.99999641
ACGACGA308.380439E-658.33333263
ACTTGCG509.822543E-1055.99999663
CGTTCGA252.3911036E-455.99999627
TCGGACG252.3911036E-455.99999644
CGATGTA252.3911036E-455.99999633
CGGTATG252.3911036E-455.99999612
CGACTAA252.3911036E-455.99999669
TCGCACG200.006952152652.548
ACGACTA407.484796E-752.539
ACCGGTC352.087564E-550.012
GCCGAAT352.087564E-550.050
GTCCGAC352.087564E-550.049
CGTCGAC352.087564E-550.07
CGTCGAA352.087564E-550.059
TACAACG352.087564E-550.059
TATACGA651.8553692E-1048.46153634
TCCGACG800.048.12550
Produced by FastQC (version 0.11.5)