FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869040
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG851.06563675E-732.94107425
GTCCTAC15500.031.8394661
AACGAAT2950.029.66092531
TCCTACA17250.028.405712
GTAGGAC15900.028.1760143
CGCGAAA500.00721161327.99991664
CGGTAAT500.00721161327.99991648
CAAGTCG6700.027.68648156
ACGAATG3200.027.34366632
TAGGACC9750.027.281974
TCGTCAA7200.027.2221460
AAGTCGT6750.026.9628857
CGCATAT657.961351E-426.92299755
CGAATGT3450.026.37673233
GCTATCG808.6737055E-526.2499225
ATACGCA959.402187E-625.78939252
AGTCGTC7200.025.277758
AATCACG8200.025.1828534
CAACGAA3800.024.86834330
CTACAGT23600.024.7668724
Produced by FastQC (version 0.11.5)