FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898496
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTATATCTACATGATAATACATAATGACCCACCAAACTCATGCATATC13840.154035187691431No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG13830.15392389059049788No Hit
ATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGG12320.13711802834959755No Hit
GTATTAGGGCTAAAATTAGGATAGATAAGATTAAGGCTAGGACACCTCCT9690.10784689080418834No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG9430.10495316617992734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTA207.925708E-570.0035139
CTAGCGT207.925708E-570.0035141
AAGTCGG150.002224303870.0035141
AGAGTCG455.456968E-1270.003540
CTAGACG150.002224794869.9996152
GTCGCTA1000.069.9996132
GTACCTA207.927893E-569.9996165
CTCGTTC207.927893E-569.9996124
CCCTCGT150.002224794869.9996144
CGTATCT800.069.9996129
ATCTACT800.069.9996145
TCCGAAC401.3460522E-1069.9996117
GGGTCAC252.840583E-669.9996168
GGGTCAA850.069.9996161
TAGGTTC1000.069.999615
GTCCTCG150.002224794869.9996112
CGTTGTA150.002224794869.9996162
GACGTGT207.927893E-569.9996135
TAGGTCG207.927893E-569.999615
TTAGACC150.002224794869.9996165
Produced by FastQC (version 0.11.5)