FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442549
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC44510.10019022862775401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA1206.548362E-1132.08298514
TAACGTA1403.965397E-1027.49975
ATTCGCG1154.9112714E-827.39131447
CGACGTA657.96136E-426.92399443
TCGACGG1606.548362E-1126.24971428
GTCCTAC31300.025.9427911
TATACTG14950.025.7522265
TAATACG1508.8402885E-1025.6663864
TTCGACG851.3095423E-424.70672641
ATTACGC851.3099425E-424.7056123
CGACTAG6950.024.6759956
CGATACG1001.400055E-524.49973360
ATTTCGT14350.024.39107542
GTAGGAC35650.024.0530343
GATTTCG15400.023.4098941
CGTCATT15300.023.33334246
TCGTACG1052.0425063E-523.3330782
GCCGTAC3250.022.6156441
TGTTACG1553.515015E-822.58039955
CCTATAC15500.022.5803993
Produced by FastQC (version 0.11.5)