HGLJYAFX2_n01_dpr176.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HGLJYAFX2_n01_dpr176.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	704112
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1359	0.19300906673938237	No Hit
CAGAAGGAGAATCAGAATTAGTATCAGGGTTTAACGTAGAATACGCAGCC	1048	0.1488399572795237	No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC	1017	0.1444372486195378	No Hit
GGATAATACTATGCCTTCCAGGCATAGTAATGTGGATATTAGGTGAGAGC	1001	0.14216488285954507	No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA	985	0.13989251709955233	No Hit
TTCTTATCCTCCCAGGATTTGGAATTATTTCACATGTAGTTACTTACTAC	875	0.12427000249960232	No Hit
GTACTGCACTAAGTATTTTAATTCGAGCAGAATTAGGTCAACCAGGTGCA	872	0.1238439339196037	No Hit
GTGTATATATCATGAAGAGAATTGTATAATTGATCATGTGACAAAAAGGG	868	0.12327584247960552	No Hit
GTTTTATGGTTAGGTTGTTTAGTTCTAGTGCGATTAGGAATCCTAATACT	861	0.1222816824596087	No Hit
ATCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGCTCTACCTCACCAT	852	0.12100347671961278	No Hit
ACCATGGAGAGTGGCAATGCTTCACCATGCTCCCTTGTATGCAAAGATAG	841	0.11944122525961778	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCGATTGACAGGGACAGCCG	837	0.1188731338196196	No Hit
CCAGTTGCGTTTGACCAACGCCAGTGCCAGCAGGCCGCTAACCATCATCA	833	0.11830504237962142	No Hit
TTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTC	816	0.11589065375962913	No Hit
GAGGAATTACAACTCATTAAAGCCAAAATTAACGTACTGATTGGGTAGTG	816	0.11589065375962913	No Hit
GCCCACCACATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTT	806	0.1144704251596337	No Hit
AACGGAATCGTTGCACGGTCGTACACGCGTTTACTTTGCGGCAGATGAAC	798	0.11333424227963733	No Hit
GGGTAGGCCATATAAGATATATAGATTATTGATCTATAATTTAACTTTGA	789	0.11205603653964141	No Hit
TATCCAGGCTGAAAAATCCCAGCAGTCCAGTTACTAATCTTTCCGCGTCT	777	0.11035176221964686	No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAGCATGAAC	742	0.10538096211966277	No Hit
ACGTACACCCAGTGAAATTTGCTCTTCACCTTCTGAATAAACAAGACGTG	728	0.10339264207966914	No Hit
TCTCAAGACATCAAGAAGAAGGAGCTACTCCCCACCACCAGCACCCAAAG	724	0.10282455063967096	No Hit
GCCCGGCGTTTCTCATTCACCAGTGCAGTCTGCTTTGCATCCGGCGTTGG	714	0.10140432203967552	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	705	0.1001261162996796	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGCG	55	0.0	70.00001	49
ACAGTCG	45	5.456968E-12	70.00001	46
AGATCGG	55	0.0	70.00001	32
GCGACTC	55	0.0	70.00001	70
CTCCGTA	45	5.456968E-12	70.00001	20
CGCGGGT	45	5.456968E-12	70.00001	31
GTCTCCG	45	5.456968E-12	70.00001	18
ATCCGAG	45	5.456968E-12	70.00001	56
ACTAGCG	55	0.0	70.00001	49
ACTAGAT	55	0.0	70.00001	54
CACTCGT	45	5.456968E-12	70.00001	34
GTGCTAA	45	5.456968E-12	70.00001	11
GGAACCG	45	5.456968E-12	70.00001	25
GTCCGTG	55	0.0	70.00001	9
GTACTAG	45	5.456968E-12	70.00001	1
ACTCGTG	55	0.0	70.00001	6
ACTCGTC	45	5.456968E-12	70.00001	35
TACTCGT	55	0.0	70.00001	5
GGGCCGA	55	0.0	70.00001	54
TCCCGCA	55	0.0	70.00001	52

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content