FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5075919
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT1154.0017767E-1133.4779762
GTAGGAC37500.023.6131293
GTCCTAC34950.023.4339681
CCTATAC13950.023.3331343
CTATACT17200.023.1974774
TATACTG19550.023.094435
CGTTACG952.783196E-422.10529144
TATACCG2850.022.1050725
TAGGACC28750.022.0345944
GTATAGG15150.021.48561
TACACTG36100.021.0385995
TATGGCG26800.021.0259416
CGTATAG1205.719965E-520.417938
TACACAG44500.020.213315
TCCTACA44450.019.9998282
CGTAACG2101.9681465E-919.99982864
TAGTACG2453.092282E-1119.9998284
ATAGGAC13850.019.9637283
GTCTAGG21250.019.765121
ATATGGC49350.019.71614315
Produced by FastQC (version 0.11.5)