FastQCFastQC Report
Wed 2 Sep 2020
HGLJYAFX2_n01_dpr174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGLJYAFX2_n01_dpr174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5980947
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC76860.128508077399783No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG69220.11573418055702549No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGA407.487124E-752.50262540
CGACGTA200.00695369452.4995527
GTTCGAC1000.052.4995511
CGATCGG506.207847E-848.9995818
CGACGCG503.4835575E-641.9996385
GTTCGCG801.4279067E-939.37466411
CGACGTT556.70394E-638.18180520
TCGAACG1051.4551915E-1136.6663553
TCGAGCG1151.8189894E-1236.52142767
ACGAATG9200.036.14099532
CCGCGTA1003.274181E-1035.00174740
CGACGGA400.00242024634.99999222
TCGACGA904.5165507E-934.99970226
TACGCCG1400.034.99975
CGAATGT9700.034.63887833
CGTCGAA1253.6379788E-1233.5997151
ACTAGCG751.4933266E-632.66638660
GCGACGT652.104773E-532.30930340
TTATACG2600.032.3074154
TATGGCG54400.031.46112616
Produced by FastQC (version 0.11.5)